文献类型：期刊文献

英文题名：A SERUM-FREE MEDIUM FOR HYBRIDOMA CELL-CULTURE

作者：CHEN, ZH; KE, YZ; CHEN, YL

年份：1993

卷号：11

期号：3

起止页码：169

外文期刊名：CYTOTECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:A1993LY11800002)】；

语种：英文

外文关键词：CELL CULTURE; HYBRIDOMA; MONOCLONAL ANTIBODY; SERUM-FREE MEDIUM

摘要：The effects of several different substances, including insulin, transferrin, ethanolamine, selenite and butyrate on the growth of murine hybridoma 2F7 cells, which secrete monoclonal antibody against small cell lung cancer, were investigated, and a serum-free medium SFM I was formulated. The effects of taurine, spermidine, progesterone and adenine on the cell growth were tested further on the basis of the medium SFM I, and a modified serum-free medium SFM II was established. On the basis of medium SFM II, the substitution tests of ferric citrate for transferrin were carried out, and it was found that transferrin could be replaced. The experiments suggested that the formulated serum-free medium was suitable for 2F7 cell growth and monoclonal antibody secretion, and thus facilitated subsequent purification of monoclonal antibody.