文献类型：期刊文献

英文题名：Biodegradation of benzene homologues in contaminated sediment of the East China Sea

作者：Li, Hui[1,2];Zhang, Qian[2];Wang, Xiao-Li[2];Ma, Xing-Yuan[1,2];Lin, Kuang-Fei[1,2];Liu, Yong-Di[1];Gu, Ji-Dong[3];Lu, Shu-Guang[1];Shi, Lei[2];Lu, Qiang[1];Shen, Ting-Ting[1]

机构：[1]E China Univ Sci & Technol, Sch Resource & Environm Engn, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, State Environm Protect Key Lab Environm Risk Asse, Shanghai Key Lab Funct Mat Chem,Sch Bioengn, Shanghai 200237, Peoples R China;[3]Univ Hong Kong, Swire Inst Marine Sci, Sch Biol Sci, Hong Kong, Hong Kong, Peoples R China

年份：2012

卷号：124

起止页码：129

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20124415623445);WOS:【SCI-EXPANDED(收录号:WOS:000311175000018)】；

基金：This research was financially supported by the National Natural Science Foundation of China (41003031, 41273109), the Specialized Research Fund for the Doctoral Program of Higher Education (20110074130002), the Shanghai Rising-Star Program (12QA1400800), the National Environmental Protection Public Welfare Science and Technology Research Program of China (200909089, 201109013), the Fundamental Research Funds for the Central Universities (WB1014004), the National Science Foundation for Postdoctoral Scientists of China (2012M510811). We also would like to thank the anonymous referees for their helpful comments on this paper.

语种：英文

外文关键词：BTEX; Biodegradation; Bacterial community; Metabolic pathway; Compatible solutes

摘要：This study focused on acclimating a microbial enrichment to biodegrade benzene, toluene, ethylbenzene and xylenes (BTEX) in a wide range of salinity. The enrichment degraded 120 mg/L toluene within 5 d in the presence of 2 M NaCl or 150 mg/L toluene within 7 d in the presence of 1-1.5 M NaCl. PCR-DGGE (polymerase chain reaction-denatured gradient gel electrophoresis) profiles demonstrated the dominant species in the enrichments distributed between five main phyla: Gammaproteobacteria, Sphingobacteriia, Prolixibacter, Flavobacteriia and Firmicutes. The Marinobacter, Prolixibacter, Balneola, Zunongwangia, Halobacillus were the dominant genus. PCR detection of genotypes involved in bacterial BETX degradation revealed that the degradation pathways contained all the known initial oxidative attack of BTEX by monooxygenase and dioxygenase. And the subsequent ring fission was catalysed by catechol 1,2-dioxygenase and catechol 2,3-dioxygenase. Nuclear magnetic resonance (NMR) spectroscopy profiles showed that the bacterial consortium adjusted the osmotic pressure by ectoine and hydroxyectoine as compatible solutes to acclimate the different salinity conditions. (C) 2012 Elsevier Ltd. All rights reserved.