文献类型：期刊文献

英文题名：Poly(vinyl alcohol) Modified Porous Graphitic Carbon Stationary Phase for Hydrophilic Interaction Liquid Chromatography

作者：Hou, Yanjie[1];Zhang, Feifang[1];Liang, Xinmiao[1];Yang, Bingcheng[1];Liu, Xiaodong[2];Dasgupta, Purnendu K.[3]

机构：[1]East China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China;[2]Thermo Fisher Sci, 445 Lakeside Dr, Sunnyvale, CA 94087 USA;[3]Univ Texas Arlington, Dept Chem & Biochem, Arlington, TX 76019 USA

年份：2016

卷号：88

期号：9

起止页码：4676

外文期刊名：ANALYTICAL CHEMISTRY

收录：;EI(收录号：20162102412448);WOS:【SCI-EXPANDED(收录号:WOS:000379636600015)】；

基金：The research was sponsored by National Natural Science Foundation of China (Grants 21322502 and 21477037). The authors gratefully acknowledge support from the outstanding young talent cultivation fund of ECUST. The participation of P.K.D. was made possible by support from the U.S. National Science Foundation through Grant CHE-1506572.

语种：英文

外文关键词：Carbon - Hydrophilicity - Liquid chromatography - Filtration - Polyvinyl alcohols - Porous materials

摘要：We report a poly(vinyl alcohol) (PVA)-coated porous graphitic carbon (PGC, Hypercarb) packing as a novel stationary phase for hydrophilic interaction liquid chromatography (HILIC). The exterior and the pores of the PGC particles are coated with a thin layer of PVA by soaking the particles in a PVA solution, filtering, and thermally cross linking the PVA. Such PVA coated PGC particles (5.7 mu m diameter), hereinafter called PVA PGC are stable at least through pH 1.0-12.7, can be made in <2 h, and exhibit different selectivity relative to six commercial HILIC phases and bare PGC. To our knowledge, this is the first fully pH-stable, completely neutral HILIC phase. Excellent efficiency stable is observed for polar analytes (similar to 70 000 and 118 000 plates/m for cytosine and resorcinol, respectively). Retention closely resembles standard HILIC behavior. Other substances can also be easily incorporated in the PVA layer; an anion exchange column can be readily made by incorporating diallyldimethylammonium chloride in the PVA coating solution. The ease of preparation without the requirement of synthetic skills or paraphernalia and the possibility of incorporating a variety of modifiers makes this a particularly versatile approach.