文献类型：期刊文献

英文题名：Electrospun poly(L-lactide-co-caprolactone)-collagen-chitosan vascular graft in a canine femoral artery model

作者：Wu, Tong[1];Jiang, Bojie[2];Wang, Yuanfei[3];Yin, Anlin[1];Huang, Chen[1];Wang, Sheng[2];Mo, Xiumei[1]

机构：[1]Donghua Univ, Coll Chem Chem Engn & Biotechnol, State Key Lab Modificat Chem Fibers & Polymer Mat, Shanghai 201620, Peoples R China;[2]Tongji Univ, Shanghai Peoples Hosp 10, Dept Emergency & Crit Care Med, Shanghai 200072, Peoples R China;[3]E China Univ Sci & Technol, Sch Resources & Environm Engn, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2015

卷号：3

期号：28

起止页码：5760

外文期刊名：JOURNAL OF MATERIALS CHEMISTRY B

收录：;EI(收录号：20152801030729);WOS:【SCI-EXPANDED(收录号:WOS:000357830600012)】；

基金：This research was supported by the National Natural Science Foundation of China (31470941, 31271035, and 51403033), the Science and Technology Commission of Shanghai Municipality (11nm0506200), PhD Programs Foundation of the Ministry of Education of China (20130075110005), light of textile project (J201404) and the Fundamental Research Funds for the Central Universities (2232014D3-15).

语种：英文

外文关键词：Biodegradability - Biomechanics - Endothelial cells - Tissue engineering - Gene expression - Chitosan - Muscle

摘要：Poly(L-lactide-co-caprolactone)-collagen-chitosan (P(LLA-CL)-COL-CS) composite grafts were electrospun in this study. Based on the test results for mechanical properties, biodegradability and in vitro cellular compatibility, the optimal weight ratio of P(LLA-CL) to COL/CS was set as 3 : 1. In vivo study was further performed in a canine femoral artery model. The results showed that the 3 : 1 grafts possessed excellent structural integrity, higher patency rate, better endothelial cell (EC) and smooth muscle cells (SMC) growth, as well as higher levels of gene and protein expression of angiogenesis-related cues than those of grafts based on P(LLA-CL). The findings confirmed that the addition of natural materials, such as collagen and chitosan, could effectively improve endothelialization, SMC incursion into the tunica media, and vascular remodeling for tissue engineering.