文献类型：专利

英文题名：Quality stabilization of anti-tumor protein medicine by expressing target protein in Escherichia coli and preparing yeast by fermentation, recycling inclusion body of Escherichia coli bacteria, and washing inclusion body of target protein

作者：MA X;MO J;SHI L;ZHU L;ZHENG W;TANG L

机构：[1]UNIV EAST CHINA SCI&TECHNOLOGY

申请号：CN102366628-A

申请日：2010-12-31

公开日：2012-03-07

语种：英文

收录：DERWENT

摘要：NOVELTY - Quality of anti-tumor protein medicine is stabilized by expressing target protein in Escherichia coli and preparing yeast by fermentation; recycling inclusion body of E. coli bacteria, washing inclusion body of target protein, subjecting target protein to strong cation column Sepharose Fast Flow chromatography purification, subjecting obtained product to anion column diethylaminoethyl cellulose agarose gel chromatography purification, recovering the target protein, and preparing recovered target protein into freeze-dried powder injection. USE - A quality stabilizing method of an anti-tumor protein medicine (claimed). ADVANTAGE - The method ensures quality of product. DETAILED DESCRIPTION - Quality of anti-tumor protein medicine is stabilized by expressing target protein in Escherichia coli and preparing yeast by fermentation, recycling inclusion body of E. coli bacteria, washing inclusion body of target protein, subjecting target protein to strong cation column Sepharose Fast Flow chromatography purification, subjecting obtained product to anion column diethylaminoethyl cellulose agarose gel chromatography purification, recovering the target protein, storing recovered target protein, and preparing stored target protein into freeze-dried powder injection. The target protein is an anti-tumor VIII mutant protein or its mutant.