文献类型：期刊文献

英文题名：Sandwich-type electrochemical aptasensor based on HMCS@PDA@AuNPs and PtCu DNs/MUN-CuO-TiO2 for ultrasensitive detection of cardiac troponin I

作者：Wang, Zhenxing[1,2,3];Zhao, Hongli[1];Chen, Kaicha[1];Cao, Shida[1];Lan, Minbo[1]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]Sinopec Maoming Petrochem Co, Maoming 525000, Peoples R China;[3]Guangdong Prov Key Lab Adv Green Lubricating Mat, Maoming 525000, Peoples R China

年份：2023

卷号：393

外文期刊名：SENSORS AND ACTUATORS B-CHEMICAL

收录：;EI(收录号：20232914400101);WOS:【SCI-EXPANDED(收录号:WOS:001058380300001)】；

基金：This work was supported by the Science and Technology Commission of Shanghai Municipality (STCSM, 20520712500; 20392002300) , and the Fundamental Research Funds for the Central Universities (JKJ01221718) .

语种：英文

外文关键词：Cardiac troponin I; Graphene-like metal oxides; Aptasensor; Hollow mesoporous carbon spheres; Signal amplification

摘要：The key to diagnosing acute myocardial infarction (AMI) lies in the sensitive and accurate analysis of cardiac troponin I (cTnI) content in human blood. Herein, in order to detect the content of cTnI, a novel sandwich-type cTnI electrochemical aptasensor was developed by utilizing dendritic platinum-copper alloy nanoparticles loaded on graphene-like CuO-TiO2 mesoporous ultrathin nanosheet (PtCu DNs/MUN-CuO-TiO2) as label material, and AuNPs loaded on polydopamine modified hollow mesoporous carbon spheres (HMCS@PDA@AuNPs) as electrode-modified material. On the one hand, with its advantages of assistance of Au and PDA coating, abundant pore structure, and large specific surface area, the HMCS@PDA@AuNPs exhibited excellent electronic conductivity but with a low catalytic capacity for the reduction of H2O2, thus enhancing the sensitivity of aptasensor. On the other hand, because of combining the structural and electrochemical performance superiority of MUN-CuO-TiO2 and PtCu DNs, such as short electron transfer distance and abundant reactive sites, the catalytic performance of obtained PtCu DNs/MUN-CuO-TiO2 towards H2O2 reduction was enhanced and realized signal amplification. The constructed electrochemical aptasensor for cTnI detection exhibited a satisfactory dynamic range from 0.01 pg/mL to 500.0 ng/mL and a lower detection limit of 2.3 fg/mL. The recommended sensor has been utilized to detect human serum samples with acceptable results, exhibiting potential for clinical application.