文献类型：期刊文献

英文题名：A new surfactin-C17 produced by Bacillus subtilis TD7 with a low critical micelle concentration and high biological activity

作者：Qin, Wan-Qi[1,2];Fei, Dan[3];Zhou, Lei[1,2];Guo, Yu-Jia[1,2];An, Shun[1,2];Gong, Ou-Hang[1,2];Wu, Yun-Yang[1,2];Liu, Jin-Feng[1,2,4];Yang, Shi-Zhong[1,2,5];Mu, Bo-Zhong[1,2,5]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[3]Jiangxi Acad Agr Sci, Inst Qual Safety & Stand Agr Prod, Nanchang 330200, Jiangxi, Peoples R China;[4]Daqing Huali Biotechnol Co Ltd, Daqing 163511, Heilongjiang, Peoples R China;[5]Shanghai Collaborat Innovat Ctr Biomfg Technol, Shanghai 200237, Peoples R China

年份：2023

卷号：47

期号：16

起止页码：7604

外文期刊名：NEW JOURNAL OF CHEMISTRY

收录：;EI(收录号：20231513869802);WOS:【SCI-EXPANDED(收录号:WOS:000960683200001)】；

基金：This work was supported by the National Key Research and Development Program (2022YFC2105200), the Fundamental Research Funds for the Central Universities of China (22221818014), and the Research Program of the State Key Laboratory of Bioreactor Engineering. The authors thank The Research Centre of Analysis and Test of East China University of Science and Technology for the help with ESI-MS and NMR analyses.

语种：英文

外文关键词：Bacteriology - Bioactivity - Biomolecules - Bioremediation - Hydrophobicity - Micelles - Palmitic acid - Solvent extraction

摘要：A new member of the surfactin family of microbial lipopeptides has been isolated from the cell broth of Bacillus subtilis TD7 using a combination of organic solvent extraction and RP-HPLC, and its primary chemical structure was determined by ESI-MS, LC-MS/MS, GC-MS, H-1-NMR, C-13-NMR and amino acid analysis. The surfactin-C-17 consists of a beta-hydroxy heptadecanoic acid chain and a heptapeptide ring with the sequence of N-Glu-Leu-Leu-Val-Asp-Leu-Leu-C, which is a new member with the longest hydrophobic chain in the surfactin family produced by B. subtilis. Compared with the reported members of surfactins-C-11 similar to 16, surfactin-C-17 showed a very low critical micelle concentration (CMC) of 4.02 x 10(-6) mol L-1 in 10 mM PBS at 25 degrees C, and a very low surface tension of 28.98 mN m(-1) at CMC (gamma(CMC)); meanwhile, it inherited a strong antibacterial activity against the Gram-positive Micrococcus luteus with a MIC value of 60 mg L-1, and a higher hemolytic activity on sheep erythrocytes. This work concluded that increasing the hydrophobic chain length of a surfactin certainly contributed to decreasing its CMC and would facilitate the development of environmentally friendly biosurfactants and extend the domain of surfactin biosurfactants to new applications such as in pharmaceutical, bioremediation and detergent industries.