文献类型：期刊文献

英文题名：Graphene quantum dots with Zn2+ and Ni2+ conjugates can cleave supercoiled DNA

作者：Liu, Fei[1];Zhang, Fangwei[2];Liu, Hui[1];Zhang, Jingyan[1];Guo, Shouwu[2]

机构：[1]East China Univ Sci & Technol, Shanghai Key Lab New Drug Design, Sch Pharm, State Key Lab Bioreactor Engn, Shanghai, Peoples R China;[2]Shanghai Jiao Tong Univ, Sch Elect Informat & Elect Engn, Shanghai, Peoples R China

年份：2016

卷号：69

期号：22

起止页码：3395

外文期刊名：JOURNAL OF COORDINATION CHEMISTRY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000386021200008)】；

基金：This work was supported by the State key laboratory of bioreactor engineering [grant number 2060204]; the Fundamental Research Funds for the Central Universities [grant number WY1514335]; 111 Project [grant number B07023]; the Shanghai Committee of Science and Technology [grant number 11DZ2260600]; the National "973 Program" of China [grant number 2014CB260411], [grant number 2015CB931801]; National Science foundation of China [grant number 11374205]; National "863" Program of China [grant number 2012AA022603].

语种：英文

外文关键词：Graphene quantum dots; DNA cleavage; Zn2+; Ni2+; complexes

摘要：Graphene quantum dots (GQDs), inheriting the superb property of graphene oxide, possess smaller lateral size and high biocompatibility, thus having potential in biomedical applications. We previously discovered that GQDs, combining with Cu2+ ions, could cleave DNA primarily through an oxidative pathway; yet, oxidative DNA cleavage is not practically preferred in biology. In this work, we explore the DNA cleavage ability of GQDs with Zn2+ and Ni2+. Zn2+ and Ni2+ alone are incapable of cleaving supercoiled DNA, but when combining with the GQDs, Zn2+ and Ni2+ exhibit DNA cleavage activity. However, the activity of these two systems is much lower than that of GQDs/Cu2+, and GQDs/Ni2+ is less active than GQDs/Zn2+. The functional mechanism of GQDs/Ni2+ and GQDs/Zn2+ is different from that of GQDs/Cu2+. The GQDs play a key role in the two systems; the redox inactive Zn2+ and Ni2+ ions assist to generate the oxidative species that eventually lead to the DNA cleavage. The current results together with our previous result indicate that GQDs together with metal ions can cleave supercoiled DNA, and their cleavage activities depend on the properties of metal ions: for redox active metal ions, metal ions play key roles, for redox inactive metal ions, GQDs are dominant. [GRAPHICS] .