文献类型：期刊文献

英文题名：Rapid initiation of guided bone regeneration driven by spatiotemporal delivery of IL-8 and BMP-2 from hierarchical MBG-based scaffold

作者：Lin, Dan[1,2];Chai, Yanjun[1,3];Ma, Yifan[1,3];Duan, Bing[1,2];Yuan, Yuan[1];Liu, Changsheng[1,2,3]

机构：[1]East China Univ Sci & Technol, Minist Educ, Engn Res Ctr Biomat, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Minist Educ, Key Lab Ultrafine Mat, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2019

卷号：196

起止页码：122

外文期刊名：BIOMATERIALS

收录：;EI(收录号：20181404969123);WOS:【SCI-EXPANDED(收录号:WOS:000459527700011)】；

基金：The authors wish to express their gratitude to the financial supports from National Natural Science Foundation of China (No. 31470924, No. 31330028 and No. 31771040), the National Natural Science Foundation of China for Innovative Research Groups (No. 51621002), Leading talents in Shanghai in 2017, 111 Project (B14018) and the Fundamental Research Funds for the Central Universities (222201718002).

语种：英文

外文关键词：Rapid initiation; BMP-2; IL-8; MBG; In situ guided bone regeneration

摘要：Initiation of endogenous repair mechanisms, including key steps of stem cell recruitment and cartilage intermediate formation in endochondral ossification, is vital to regeneration of large bone defects. To biomimetically promote a rapid initiation and ensuing osteogenic stimulation, exogenous chemokine IL 8 and growth factor BMP-2 were orchestrated in a mesoporous bioactive glass (MBG)-based spatiotemporal delivery system, to achieve a rapid release of IL-8 followed by a long-term sustained release of BMP-2. The synergistic effect of IL-8 and BMP-2 on initiation stage of bone healing and underlying mechanism were thoroughly investigated in vitro and in vivo. Intriguingly, apart from its superiority in stem cell recruitment to BMP-2, IL-8 not only endowed a histological "prep-state" of endochondral ossification by up-regulating chondrogenic genes and inducing the formation of extensive cartilage tissues, facilitating rapid bone transformation by BMP-2, but also triggered a cellular "prep-state" with high expression of BMP receptors, enhancing the osteoinductivity of BMP-2. With the spatiotemporal delivery system, orchestrated signal stimuli of IL-8 and BMP-2 induced a rapid initiation including efficient stem cell recruitment and a "chondrogeniciosteogenic balance" at the first stage of endochondral ossification, and the scaffold facilitated sufficient osteoconductivity, together resulting in early extensive bone mineralization and an advanced regeneration throughout the repair of large bone defect. We believe this new idea could provide insights toward designing bone-repairing biomaterials with higher regenerative efficiency. (C) 2017 Elsevier Ltd. All rights reserved.