文献类型：期刊文献

中文题名：溶藻弧菌铁载体合成及外膜蛋白表达的研究

英文题名：Growth,Siderophore Production and Outer Membrane Protein Expression of Vibrio alginolyticus by Iron Regulation

作者：王蓬勃[1];马悦[1];刘琴[1];张元兴[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2006

卷号：33

期号：2

起止页码：48

中文期刊名：微生物学通报

外文期刊名：Microbiology China

收录：CSTPCD;;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：溶藻弧菌;铁载体;外膜蛋白

外文关键词：Vibrio alginolyticus, Siderophore, Outer membrane protein

摘要：初步研究了海洋动物病原菌溶藻弧菌的铁摄取机制。溶藻弧菌能够在高浓度铁螯合剂2-2二联吡啶的培养基中存活。在限铁环境中,溶藻弧菌生长受到抑制,补加铁可以消除这种抑制作用。通过铁载体定量检测,发现分离于发病鱼体的溶藻弧菌MVP01产铁载体量大于分离于海水的菌株No·1·1587。互补实验证明溶藻弧菌的铁载体粗提物能够被铁载体合成缺陷的大肠杆菌突变株AN93利用。在铁限制培养环境中,溶藻弧菌合成了约80kD铁调控外膜蛋白。铁摄取系统在溶藻弧菌的生存和致病性方面,都有重要的作用。
Iron uptake mechanism of Vibrio alglnolyticus was primarily investigated, V. alginolyticus could surrive in the medium with high-concentration iron chelator. The strain of V. alginolyticus isolated from diseased fish produced more siderophore than that from marine environment. The extract of siderophore from V. alginolyticus could stimulate the growth of Escherichia coil mutant AN93. Under iron limitation, the growth rate was decreased and several outer membrane proteins were induced. Adding iron into the iron-limited medium the normal growth could be recovered.