文献类型：期刊文献

英文题名：Late-stage difluoromethylation leading to a self-immobilizing fluorogenic probe for the visualization of enzyme activities in live cells

作者：Jiang, Jialing[1];Tan, Qingwei[1];Zhao, Shuxuan[1];Song, Heng[1];Hu, Liqiang[1];Xie, Hexin[1]

机构：[1]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2019

卷号：55

期号：99

起止页码：15000

外文期刊名：CHEMICAL COMMUNICATIONS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000502103100027)】；

基金：This study was financially supported by NSFC-BRICS (81861148020), the Research Program of State Key Laboratory of Bioreactor Engineering, and the Recruitment Program for Young Professionals.

语种：英文

摘要：Reported herein is a novel p-quinone methide-based self-immobilizing fluorogenic probe for the visualization of beta-galactosidase activities in live cells. This easily prepared imaging reagent massively increases the fluorescence intensity and covalently links to the activation site with high efficiency upon enzymatic trigger.