文献类型：期刊文献

中文题名：小鼠胶源神经营养因子基因的克隆及其性质研究

英文题名：Cloning and Characterization of Mouse Glial Cell Linederived Neurotrophic Growth Factor (GDNF)

作者：王富军[1];赵健[2,1]

机构：[1]国立新加坡大学生化系;[2]华东理工大学应用生物学系

年份：1998

卷号：30

期号：4

起止页码：375

中文期刊名：生物化学与生物物理学报

收录：CSTPCD;;Scopus;CSCD:【CSCD2011_2012】；PubMed;

语种：中文

中文关键词：小鼠;基因克隆;表达;神经营养因子;GDNF

外文关键词：Mouse; gene cloning and expression; growth factor; GDNF

摘要：本文从成年小鼠脑基因库中克隆了两种独特的胶源神经营养因子（ｇｌｉａｌｃｅｌｌｉｎｅ－ｄｅｒｉｖｅｄｎｅｕｒｏｔｒｏｐｈｉｃｇｒｏｗｔｈｆａｃｔｏｒ，ＧＤＮＦ）转录产物（ＧＤＮＦ－α和ＧＤＮＦ－β）。ＧＤＮＦ－β除了在开放阅读框架区（ＯＲＦ）有７８ｂｐ的缺失外，其余与ＧＤＮＦ－α相同。由小鼠ＧＤＮＦ－α基因推导得的氨基酸顺序与大鼠和人的氨基酸顺序具有高度同源性。对小鼠不同组织的反转录ＰＣＲ（ＲＴ－ＰＣＲ）实验表明在这些组织中广泛存在着这两种形式的ＧＤＮＦ。ＧＤＮＦ－α至少由两个外显子编码组成。
We have cloned two unique forms of glial cell linederived neurotrophic growth factor transcripts (GDNFα and GDNFβ) from adult mouse brain. The GDNFβ form is similar to GDNFα except for a 78 bp deletion in the ORF. The deduced amino acid sequences of the mouse GDNFα are highly homologous to those of rat and human GDNF. Reversetranscription PCR from various mouse tissues showed extensive expression of both forms of GDNF. GDNFα is coded by at least two distinct exons. The 3′UTR is entirely encoded in a single exon.