文献类型：期刊文献

英文题名：Azobenzene-diamides as Photopharmacological Ligands for Insect Ryanodine Receptor

作者：Wang, Long[1];Chen, Meijun[1];Xia, Shanshan[1];Xu, Zhiping[1];Li, Yuxin[3];Shao, Xusheng[1,2]

机构：[1]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[3]Nankai Univ, Inst Elementoorgan Chem, State Key Lab Elementoorgan Chem, Tianjin 300071, Peoples R China

年份：2020

卷号：68

期号：49

起止页码：14409

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：;EI(收录号：20205109663787);WOS:【SCI-EXPANDED(收录号:WOS:000599505900011)】；

基金：This work was financially supported by the National Key Research and Development Program of China (2018YFD0200100), the National Natural Science Foundation of China (No. 21877039), and the Innovation Program of Shanghai Municipal Education Commission (2017-01-07-00-02-E00037).

语种：英文

外文关键词：photopharmacology; ryanodine receptor; azobenzene; chlorantraniliprole; insect

摘要：Photoresponsive ligands are powerful tool compounds for studying receptor function with spatiotemporal resolution. However, to the best of our knowledge, such a ligand is not available for the ryanodine receptor (RyR). Herein, we present a photochromic ligand (PCL) for insect RyR by decorating chlorantraniliprole (CHL) with photoswitchable azobenzene (AB). We demonstrated that one potent ligand, named ABCHL13, shows light-induced reversible trans-cis isomerization and 3.5-fold insecticidal activity decrease toward oriental armyworm (Mythimna separata) after UV-light irradiation, that is, trans-ABCH13 has higher activity than the cis-ABCH13. ABCHL13 enables optical control over intracellular Ca2+ release in dorsal unpaired median (DUM) neurons of M. separata and American cockroach (Periplaneta americana) and cardiac function of P. americana. Our results provide a first photopharmacological toolkit that is applicable to light-dependent regulation of RyR and heart beating.