文献类型：期刊文献

英文题名：Genome-Based Discovery of Enantiomeric Pentacyclic Sesterterpenes Catalyzed by Fungal Bifunctional Terpene Synthases

作者：Jiang, Lan[1];Zhang, Xue[1];Sato, Yuya[2];Zhu, Guoliang[1];Minami, Atsushi[2];Zhang, Weiyan[1];Ozaki, Taro[2];Zhu, Bin[1,3,4];Wang, Zhixin[1];Wang, Xinye[1];Lv, Kangjie[1];Zhang, Jingyu[1];Wang, Yongheng[5,6];Gao, Shushan[7];Liu, Chengwei[8];Hsiang, Tom[9];Zhang, Lixin[1];Oikawa, Hideaki[2];Liu, Xueting[1]

机构：[1]East China Univ Sci Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Hokkaido Univ, Fac Sci, Dept Chem, Sapporo, Hokkaido 0600810, Japan;[3]East China Univ Sci & Technol, Engn Res Ctr Pharmaceut Proc Chem, Minist Educ, Shanghai 200237, Peoples R China;[4]East China Univ Sci & Technol, Lab Pharmaceut Crystal Engn & Technol, Sch Pharm, Shanghai 200237, Peoples R China;[5]Jinan Univ, Coll Pharm, Inst Tradit Chinese Med & Nat Prod, Guangzhou 510632, Peoples R China;[6]Jinan Univ, Guangdong Prov Key Lab Pharmacodynam Constituents, Guangzhou 510632, Peoples R China;[7]Chinese Acad Sci, Key Lab Pathogen Microbiol & Immunol, Inst Microbiol, Beijing 100101, Peoples R China;[8]Northeast Forestry Univ, Coll Life Sci, Key Lab Saline Alkali Vegetat Ecol Restorat, Minist Educ, Harbin 150040, Peoples R China;[9]Univ Guelph, Sch Environm Sci, Guelph, ON N1G 2W1, Canada

年份：2021

卷号：23

期号：12

起止页码：4645

外文期刊名：ORGANIC LETTERS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000664333200028)】；

基金：We sincerely acknowledge Prof. Jun-ichi Maruyama from the University of Tokyo for the kind provision of A. oryzae NSAR1; Prof. Katsuya Gomi from Tohoku University for providing vectors pUARA2 and pUSA2; and Dr. K. Narita, Ms. A. Sato, and Mr. H. Yamamoto from Hokkaido University for preliminary experiments. We gratefully acknowledge the financial support from the National Key Research and Development Program of China (2020YFA090032 and 2019YFA0906200), the National Natural Science Foundation of China (21907031, 81903529, 21977029, and 21877038), the Open Project Funding of the State Key Laboratory of Bioreactor Engineering, the 111 Project (B18022), the Shanghai Science and Technology Commission (18JC1411900), and the Japan Society for the Promotion of Science (Grants JP16H06446 and 19H02891). Genome sequencing and assembly of in-house genomes were supported by funding from the Natural Science and Engineering Research Council of Canada to T.H.

语种：英文

摘要：Genome-based discovery of two previously unreported fungal bifunctional terpene synthases (BFTSs) from phytopathogenic fungi are reported: FoFS catalyzing the formation of fusoxypenes A-C (1-3) and (-)-astellatene (4) and AtAS capable of synthesizing preaspterpenacid I (6). Interestingly, FoFS and AtAS catalyzed the formation of enantiomeric sesterterpenes with a 5-6-7-3-5 ring system. C22-oxidative modification of preaspterpenacid I by AtP450 was characterized as well. Plausible cyclization pathways of the fusoxypenes were illustrated by DFT calculations.