文献类型：期刊文献

中文题名：用国产生物反应器大规模高密度培养Vero细胞

英文题名：Large-scale Culture of Vero Cells in High Density by Using CellCul-20 Bioreactor

作者：董树沛[1];陈因良[1];顾小华[1];严春[1];宋家骊[2];陈列胜[2];陈文兰[2]

机构：[1]华东化工学院生化工程研究所;[2]卫生部上海生物制品研究所;

年份：1992

卷号：8

期号：4

起止页码：389

中文期刊名：生物工程学报

外文期刊名：Chinese Journal of Biotechnology

收录：CSTPCD;;Scopus;北大核心:【北大核心1992】；CSCD:【CSCD2011_2012】；PubMed;

语种：中文

中文关键词：Vero细胞;细胞培养;生物反应器

外文关键词：Vero cell; microcarrier; cell culture; bioreactor.

摘要：本文考察了在2.5LCelliGen细胞培养器和国产20LCellCul-20细胞培养生物反应器中采用微载体技术培养细胞的情况。分析了用CellCul-20细胞培养生物反应器进行大规模培养时细胞的生长、代谢规律,研究了从2.5L扩大到20L规模的细胞转移条件。采用微载体球间直接转移技术,提高了接种效率,减少了接种步骤和污染机会。当国产GT-25微载体用尾为5g/L,采用连续灌注工艺培养Vero细胞,在国产20L CellCul-20细胞培养生物反应器中,连续培养5天,细胞数增加7倍,细胞密度超过1.0×10~7cells/ml。本文开发的细胞培养工艺,对于中试及工业规模的动物细胞大量培养具有一定的指导意义。
High density Vero cells were cultivated in a 20 liter CellCul-20 homemade bioreactor and a 2.5 liter CelliGen. Also, the cell growth, its metabolism and technique of cell transfer and scale-up of culture volume were investigated. GT-2 microcarriers were used and beads to beads transfer technique was employed. It was shown that the efficiency of inoculation was increased and oparating steps and risks of contamination were decreased in this experiment. The cell density was higher than 1.0×10~7 cells/ml and was 7 times higher in comparing with the inoculation number after 5 days' perfusion cultivation. Obviously, this work is significant for large-scale culture of animal cells in pilot and industrial scales.