文献类型：期刊文献

英文题名：Combining metabolic engineering and adaptive evolution to enhance the production of dihydroxyacetone from glycerol by Gluconobacter oxydans in a low-cost way

作者：Lu, Leifang[1];Wei, Liujing[1];Zhu, Kun[1];Wei, Dongzhi[1];Hua, Qiang[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2012

卷号：117

起止页码：317

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000306208900043)】；

基金：This study was financially supported by the Natural Science Foundation of Shanghai (11ZR1408100), National Basic Research Program of China (973 Program) (2012CB721101), National Special Fund for State Key Laboratory of Bioreactor Engineering (2060204), and partially supported by Shanghai Leading Academic Discipline Project (B505).

语种：英文

外文关键词：Gluconobacter oxydans; Sorbitol dehydrogenase; Adaptive evolution; Dihydroxyacetone; Biotransformation

摘要：Gluconobacter oxydans can rapidly and effectively transform glycerol to dihydroxyacetone (DNA) by membrane-bound quinoprotein sorbitol dehydrogenase (mSLDH). Two mutant strains of GDHE Delta adh pBBR-(tufB)sldAB and GDHE Delta adh pBBR-sldAB derived from the GDHE strain were constructed for the enhancement of DHA production. Growth performances of both strains were largely improved after adaptively growing in the medium with glucose as the sole carbon source. The resulting GAT and CAN strains exhibited better catalytic property than the GDHE strain in the presence of a high concentration of glycerol. All strains of GDHE, GAT and CAN cultivated on glucose showed enhanced catalytic capacity than those grown on sorbitol, indicating a favorable prospect of using glucose as carbon source to reduce the cost in industrial production. It was also the first time to reveal that the expression level of the sldAB gene in glucose-growing strains were higher than that of the strains cultivated on sorbitol. (C) 2012 Elsevier Ltd. All rights reserved.