文献类型：期刊文献

中文题名：八角茴香水溶性成分HPLC指纹图谱及莽草酸的定量研究

英文题名：HPLC fingerprint of water-soluble constituents of Illicium verum Hook.f.and qualitative analysis of shikimic acid

作者：张敏[1];米佳宁[2];杨晓岚[1];王月荣[2];章弘扬[2];胡坪[2]

机构：[1]华东理工大学药学院中药现代化工程中心,上海200237;[2]华东理工大学化学与分子工程学院,上海200237

年份：2011

卷号：45

期号：12

起止页码：91

中文期刊名：上海中医药杂志

外文期刊名：Shanghai Journal of Traditional Chinese Medicine

收录：CSTPCD

基金：国家自然科学基金项目(21006023)

语种：中文

中文关键词：八角茴香;HPLC;指纹图谱;莽草酸;定量分析

外文关键词：lllicium verum ; HPLC ; fingerprint; shikimic acid ; quantitative analysis

摘要：目的建立八角茴香水溶性成分的HPLC指纹图谱方法及莽草酸的快速定量分析方法。方法采用Waters XBirdge C_(18)色谱柱(250mm×4.6mm,5μm);流动相为0.05%磷酸水溶液(A相)-乙腈(B相),梯度洗脱;柱温25℃;流速0.5ml/min;检测波长210nm、254nm。结果对18批八角茴香药材和2批莽草及不同部位进行测定,建立了八角茴香水溶性成分HPIC指纹图谱方法和莽草酸快速定量分析方法,并对不同来源、等级和部位的八角茴香中莽草酸含量进行了分析。此外,将所建立的方法用于八角茴香伪品莽草的分析研究。结论此方法简便、可靠,为建立八角茴香的质量标准提供了依据。
Objective To establish an analysis method of high performance liquid chromatography fingerprint of the water-soluble constituents from lllicium verum Hook. f. and a quick quantitative method of detecting shikimic acid. Method A Waters XBirdge C18 column （250 mm × 4.6 mm, 5 um） was used; the mobile phase was consisted of 0.05 % n3 PO4 （ A ） -acetonitrile （ B ） with gradient elution, the column temperature was set at 25 ℃, the flow rate was 0.5 ml/min and the detection wavelength was 210 nm and 254 nm. Results The fingerprint and the determination of shikimic acid of 18 populations of lllicium verum Hook. f. and two populations of lllicium lanceolatum A. C. Smith from different sources and between different parts were analyzed by the methods established. Conclusion The methods were proved to be simple, reliable, and useful for quality control of lllicium verum Hook. f..