文献类型：期刊文献

英文题名：Empowering probiotics with high xanthine transport for effective hyperuricemia management

作者：Zou, Zhen-Ping[1];Li, Ju-Ling[1];Zhang, Yi-Fan[1];Zhou, Ying[1];Ye, Bang-Ce[1]

机构：[1]East China Univ Sci & Technol, Inst Engn Biol & Hlth, Lab Biosyst & Microanal, State Key Lab Bioreactor Engn, Shanghai, Peoples R China

年份：2024

卷号：16

期号：1

外文期刊名：GUT MICROBES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001314431600001)】；

基金：This work was sponsored by the National Key Research and Development Program of China (2023YFF1204500), the National Natural Science Foundation of China (22134003), and China Postdoctoral Science Foundation (2023M741176).

语种：英文

外文关键词：Engineered probiotics; Hyperuricemia; intestinal purine

摘要：Hyperuricemia, a prevalent metabolic disorder, poses a susceptibility to various complications. The conventional pharmacotherapeutic approaches for hyperuricemia often entail notable adverse effects, posing substantial clinical challenges. Hence, the imperative lies in the development of novel, safe and effective strategies for preventing and treating hyperuricemia. Here, we developed a probiotic Escherichia coli Nissle 1917 strain, designated as YES301, which contains a rationally designed xanthine importer XanQ, enabling efficient uptake of xanthine and hypoxanthine, consequently leading to reduced serum uric acid concentrations and amelioration of renal impairments in a murine model of hyperuricemia. Importantly, YES301 exhibited a therapeutic efficacy comparable to allopurinol, a conventional uric acid-lowering agent, and manifesting fewer adverse effects and enhanced biosafety. These findings highlight the promising potential of engineered probiotics in the management of hyperuricemia through reducing intestinal purine levels.