文献类型：期刊文献

英文题名：Quantitatively Mapping Cellular Viscosity with Detailed Organelle Information via a Designed PET Fluorescent Probe

作者：Liu, Tianyu[1,2];Liu, Xiaogang[3];Spring, David R.[4];Qian, Xuhong[5];Cui, Jingnan[2];Xu, Zhaochao[1]

机构：[1]Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China;[2]Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116012, Peoples R China;[3]Univ Cambridge, Dept Phys, Cavendish Lab, Cambridge CB2 1TN, England;[4]Univ Cambridge, Dept Chem, Cambridge CB2 1TN, England;[5]E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China

年份：2014

卷号：4

外文期刊名：SCIENTIFIC REPORTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000337889600002)】；

基金：We are thankful for financial support from the National Natural Science Foundation of China (21276251), Ministry of Human Resources and Social Security of PRC, the 100 talents program funded by Chinese Academy of Sciences, State Key Laboratory of Fine Chemicals of China (KF1105), and the Herchel Smith Fund. D. R. S. is supported by the EPSRC, ERC, BBSRC, MRC and Wellcome Trust.

语种：英文

摘要：Viscosity is a fundamental physical parameter that influences diffusion in biological processes. The distribution of intracellular viscosity is highly heterogeneous, and it is challenging to obtain a full map of cellular viscosity with detailed organelle information. In this work, we report 1 as the first fluorescent viscosity probe which is able to quantitatively map cellular viscosity with detailed organelle information based on the PET mechanism. This probe exhibited a significant ratiometric fluorescence intensity enhancement as solvent viscosity increases. The emission intensity increase was attributed to combined effects of the inhibition of PET due to restricted conformational access (favorable for FRET, but not for PET), and the decreased PET efficiency caused by viscosity-dependent twisted intramolecular charge transfer (TICT). A full map of subcellular viscosity was successfully constructed via fluorescent ratiometric detection and fluorescence lifetime imaging; it was found that lysosomal regions in a cell possess the highest viscosity, followed by mitochondrial regions.