文献类型：期刊文献

中文题名：Monitoring NAD(H) and NADP(H) dynamics during organismal development with genetically encoded fluorescent biosensors

作者：Ting Li[1,2];Yejun Zou[1,2];Shuning Liu[1,2];Yi Yang[1];Zhuo Zhang[1,2,3];Yuzheng Zhao[1,2,3]

机构：[1]Optogenetics&Synthetic Biology Interdisciplinary Research Center,State Key Laboratory of Bioreactor Engineering,Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism,East China University of Science and Technology,130 Mei Long Road,Shanghai 200237,China;[2]Shanghai Key Laboratory of New Drug Design,School of Pharmacy,East China University of Science and Technology,130 Mei Long Road,Shanghai 200237,China;[3]Research Unit of New Techniques for Live-cell Metabolic Imaging,Chinese Academy of Medical Sciences,Beijing,China

年份：2022

卷号：11

期号：1

起止页码：44

中文期刊名：Cell Regeneration

外文期刊名：细胞再生（英文）

收录：Scopus

基金：This research is supported by National Key Research and Development Program of China(2019YFA0904800 to Y.Zhao);NSFC(32030065,92049304,32121005 to Y.Zhao,31901033 to T.L.);Research Unit of New Techniques for Live-cell Metabolic Imaging(Chinese Academy of Medical Sciences,2019RU01,2019-I2M-5-013 to Y.Zhao);Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism(Y.Zhao);Innovative research team of high-level local universities in Shanghai,the Shanghai Science and Technology Commission(19YF1411300 to T.L.);China Postdoctoral Science Foundation(2019 M651413 to T.L.);the State Key Laboratory of Bioreactor Engineering,the Fundamental Research Funds for the Central Universities.

语种：英文

中文关键词：Cell metabolism;NAD(H)and NADP(H);Genetically encoded fluorescent sensors;Real-time monitoring;Organismal development

摘要：Cell metabolism plays vital roles in organismal development,but it has been much less studied than transcriptional and epigenetic control of developmental programs.The difficulty might be largely attributed to the lack of in situ metabolite assays.Genetically encoded fluorescent sensors are powerful tools for noninvasive metabolic monitoring in living cells and in vivo by highly spatiotemporal visualization.Among all living organisms,the NAD(H)and NADP(H)pools are essential for maintaining redox homeostasis and for modulating cellular metabolism.Here,we introduce NAD(H)and NADP(H)biosensors,present example assays in developing organisms,and describe promising prospects for how sensors contribute to developmental biology research.