文献类型：期刊文献

英文题名：Long-wavelength fluorescent boronate probes for the detection and intracellular imaging of peroxynitrite

作者：Sedgwick, Adam C.[1];Han, Hai-Hao[2,3];Gardiner, Jordan E.[1];Bull, Steven D.[1];He, Xiao-Peng[2,3];James, Tony D.[1]

机构：[1]Univ Bath, Dept Chem, Bath BA2 7AY, Avon, England;[2]East China Univ Sci & Technol, Key Lab Adv Mat, 130 Meilong Rd, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2017

卷号：53

期号：95

起止页码：12822

外文期刊名：CHEMICAL COMMUNICATIONS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000423912400024)】；

基金：We would like to thank the EPSRC and the University of Bath for funding. A. C. S. and J. E. G. thank the EPSRC for studentships. T. D. J. wishes to thank the Royal Society for a Wolfson Research Merit Award. NMR characterisation facilities were provided through the Chemical Characterisation and Analysis Facility (CCAF) at the University of Bath (www.bath.ac.uk/ccaf). The EPSRC UK National Mass Spectrometry Facility at Swansea University is thanked for analyses. X.-P. He thanks the National Natural Science Foundation of China (21722801 and 21572058), the Science and Technology Commission of Shanghai Municipality (15540723800), the Fundamental Research Funds for the Central Universities (222201717003) and the Shanghai Rising-Star Program (16QA1401400) (to X.-P. He) for financial support. The Catalysis And Sensing for our Environment (CASE) network is thanked for research exchange opportunities. T. D. J. thanks ECUST for a guest professorship. Professors Jia Li and Yi Zang are thanked for their helpful advice on the cellular experiments. All data supporting this study are provided as supplementary information accompanying this paper.

语种：英文

摘要：Two boronate fluorescent probes have been developed for the detection of peroxynitrite (TCFB1 and TCFB2). TCFB1 was shown to have a low sensitvity towards peroxynitrite and have a poor solubility in aqueous solution whereas TCFB2 demonstrated high sensitivity towards peroxynitrite and mitochondria localisation with the ability to detect exogenous and endogenous peroxynitrite in live cells (Hep-G2, RAW 264.7, HeLa and A459).