文献类型：期刊文献

中文题名：产脂肪酶菌株筛选及柠檬酸杆菌产酶条件优化

英文题名：Screening of Lipase-Producing Bacteria and Conditions Optimization of Lipase Production by Citrobacter sp.

作者：张晶晶[1];刘金峰[1];牟伯中[1];杨世忠[1]

机构：[1]华东理工大学化学系,上海200237

年份：2015

卷号：35

期号：2

起止页码：85

中文期刊名：微生物学杂志

外文期刊名：Journal of Microbiology

收录：CSTPCD;;CSCD:【CSCD_E2015_2016】；

基金：国家自然科学基金项目(31200101);科技部"863"计划项目(2013AA064403)

语种：中文

中文关键词：脂肪酶;产酶条件优化;柠檬酸杆菌

外文关键词：lipase; conditions optimization of enzyme production; Citrobacter sp.

摘要：脂肪酶可以催化甘油三酯水解成脂肪酸和甘油,已广泛应用在工业领域,而获得产酶微生物是研究的基础。采用油脂平板法筛选出1株脂肪酶产生菌。经16S rRNA序列分析可知,该菌株属于柠檬酸杆菌(Citrobacter werkman and Gillen)。单因素试验对其进行产酶条件优化,优化后产酶条件(g/L):淀粉2.0,KH2PO41.0,K2HPO4·3H2O 2.2,(NH4)2SO41.0,Mg SO4·7H2O 0.1,牛肉膏2.0,橄榄油10.0mL,pH 7.5,每升接种量为15mL,37℃培养43 h。获得最大酶活为384 U/m L,是优化前的13倍。可以利用该菌制备脂肪酶。
Lipase can catalyze hydrolysis of triglyceride into fatty acids and glycerol,and has been widely applied in industries,and to obtain lipase-producing microorganisms is the basis of the study.A novel lipase-producing microbe was isolated from lipid-contaminated soil samples using plate-screening method.It was purified and identified as Citrobacter sp.through 16 S rRNA sequence analysis.The enzyme production condition was optimized by single factor experiment.The optimum culture condition in a liter medium was 2.0（g / L,the same below） of soluble starch,1.0KH2PO4,2.2 K2HPO4·3H2O,1.0（NH4）2SO4,0.1 MgS O4·7H2O,2.0 beef extract,olive oil 10.0 mL,pH 7.5 and 15 mL of inoculum,culture for 43 h at 37 °C in shaker.The obtained enzymatic activity was 384 U / mL at the highest,and it was 13-fold of that before the optimization.This strain could be used to produce lipase.