文献类型：期刊文献

中文题名：乳酸对重组CHO细胞生长代谢及EPO表达的影响

英文题名：EFFECTS OF LACTATE ON GROWTH,METABOLISM AND EPO EXPRESSION OF RECOMBINANT CHO CELLS

作者：孙祥明[1];张元兴[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2002

卷号：53

期号：10

起止页码：1034

中文期刊名：化工学报

外文期刊名：CIESC Journal

收录：CSTPCD;;EI(收录号：2003037327796);Scopus;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：重组CHO细胞;生长代谢;乳酸;渗透压;促红细胞生长素;细胞培养;重组动物细胞

外文关键词：Glucose;Growth kinetics;Metabolism;Osmosis;Production

摘要：利用脉冲实验方法研究了乳酸和渗透压对重组CHO细胞生长代谢和EPO表达的影响 ,证明乳酸分子本身对重组CHO细胞的生长代谢和产物表达没有明显的影响 .乳酸浓度从 0增加到 6 9mmol·L-1对应于渗透压从 30 0增加到 4 2 0mOsm ,细胞的比生长速率下降了 33% ,葡萄糖和谷氨酰胺的比消耗速率均增加了 2 3% ,细胞对葡萄糖和谷氨酰胺的得率系数分别下降了 4 5 %和 4 7% ,乳酸的比生成速率则保持恒定 ,表明在高渗透压下营养物的利用更倾向于能量代谢过程 ,好氧能量代谢过程所占的比例随着渗透压的增加而增加 ;另一方面 ,氨对谷氨酰胺的得率系数增加了 4 4 % ,丙氨酸对谷氨酰胺的得率系数下降了 2 4 % ,表明谷氨酰胺经谷氨酸脱氢酶途径的代谢流量随着渗透压的增加而增加 .
The effects of lactate and osmolarity on the growth, metabolism and EPO expression of recombinant CHO cells were investigated by pulse cultures with different lactate concentrations and osmolarity. It was demonstrated that lactate moleule itself had little effect on the cell culture. With the increase of lactate concentration from 0 to 69 mmol·L-1, corresponding to the increase of osmolarity from 300 to 420 mOsm, the specific growth rate of recombinant CHO cells was reduced by 33%, the specific consumption rates of glucose and glutamine both increased by 23%, the yields of cell growth to glucose consumption and glutamine consumption were reduced by 45% and 47%, respectively, and the specific production rates of lactate almost kept constant, showing that more nutrient was consumed via aerobic metabolism pathway for more energy production to meet the increasing demand of maintaining energy of recombinant CHO cells at high osmolarity. The yield of ammonia production to glutamine consumption increased with the increase of osmolarity, while the yield of alanine was reduced. It was shown that the flux of glutamine consumed via glutamate dehydrogenase pathway increased in response to the increase of osmolarity. On the other hand, EPO specific production rates decreased with the increase of osmolarity because of lower specific growth rate.