文献类型：期刊文献

英文题名：Schiff Base Reaction in a Living Cell: In Situ Synthesis of a Hollow Covalent Organic Polymer To Regulate Biological Functions

作者：Xu, Han-Bin[1];Chen, Hua-Ying[1];Lv, Jian[1];Chen, Bin-Bin[1,2];Zhou, Ze-Rui[1];Chang, Shuai[1];Gao, Ya-Ting[1];Huang, Wen-Fei[1];Ye, Ming-Jie[1];Cheng, Zi-Jian[1];Hafez, Mahmoud Elsayed[1,3];Qian, Ruo-Can[1];Li, Da-Wei[1]

机构：[1]East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr, Frontiers Sci Ctr Materiobiol & Dynam Chem, Sch Chem & Mol Engn,Key Lab Adv Mat,Shanghai Key L, Shanghai 200237, Peoples R China;[2]Chinese Univ Hong Kong, Shenzhen Inst Aggregate Sci & Technol, Sch Sci & Engn, Shenzhen 518172, Guangdong, Peoples R China;[3]Beni Suef Univ, Fac Sci, Dept Chem, Bani Suwayf 62511, Egypt

年份：2023

卷号：62

期号：44

外文期刊名：ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

收录：;EI(收录号：20234014821100);WOS:【SCI-EXPANDED(收录号:WOS:001069747200001)】；

基金：This work was financially supported by the National Natural Science Foundation of China (21974046, 22176058, and 21977031), Science and Technology Commission of Shanghai Municipality (2018SHZDZX03, 22ZR1416800, and 23ZR1416100), the Program of Introducing Talents of Discipline to Universities (B16017), and the Fundamental Research Funds for the Central Universities (222201717003). We thank the Research Center of Analysis and Test of East China University of Science and Technology for help with the characterization.

语种：英文

外文关键词：Biological Function Regulation; Hollow Covalent Organic Polymers; Intracellular Schiff Base Reaction; in Situ Biosynthesis

摘要：Artificially performing chemical reactions in living biosystems to attain various physiological aims remains an intriguing but very challenging task. In this study, the Schiff base reaction was conducted in cells using Sc(OTf)3 as a catalyst, enabling the in situ synthesis of a hollow covalent organic polymer (HCOP) without external stimuli. The reversible Schiff base reaction mediated intracellular Oswald ripening endows the HCOP with a spherical, hollow porous structure and a large specific surface area. The intracellularly generated HCOP reduced cellular motility by restraining actin polymerization, which consequently induced mitochondrial deactivation, apoptosis, and necroptosis. The presented intracellular synthesis system inspired by the Schiff base reaction has strong potential to regulate cell fate and biological functions, opening up a new strategic possibility for intervening in cellular behavior. A Schiff base reaction has been artificially conducted in living cells and enabled the in situ synthesis of a hollow covalent organic polymer (HCOP) that inhibited the polymerization of actin filaments and consequently induced mitochondrial deactivation, apoptosis, and necroptosis. The proposed intracellular synthesis approach may provide an efficient strategy to regulate cellular behavior and biological functions.image