文献类型：期刊文献

中文题名：羰基生物还原法合成手性醇的研究进展

英文题名：Advances in synthesis of chiral alcohols by carbonyl bioreduction

作者：郁惠蕾[1];黄磊[1];倪燕[1];许国超[1];许建和[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2013

卷号：11

期号：3

起止页码：71

中文期刊名：生物加工过程

外文期刊名：Chinese Journal of Bioprocess Engineering

收录：CSTPCD;;CSCD:【CSCD_E2013_2014】；

基金：国家自然科学基金(20902023)

语种：中文

中文关键词：不对称还原;生物催化;羰基还原酶;手性醇;基因组挖掘

外文关键词：asymmetric reduction ; biocatalysis ; carbonyl reductases ; chiral alcohols ; genome mining

摘要：手性醇是合成许多光学活性药物、农用化学品及其他精细化学品的关键手性砌块。羰基生物还原法理论上可实现100%转化率,且反应条件温和,对环境十分友好,被普遍认为是生产手性醇的绿色、高效途径。综述了近年来利用生物信息学、高通量筛选和蛋白质工程的发展对新型、高效生物催化剂开发的影响,特别是利用相关技术手段开发羰基还原酶的进展。
Enantiopure secondary alcohols, as key intermediates, play an essential role in pharmaceutical, agrochemical, and chemical industries. Biocatalytic ketone reduction is being generally regarded as a green and effective access to enantiopure alcohols, due to mildness of reaction conditions and friendliness to environment. An increasing number of novel and robust enzymes become more easily accessible as a result of the ongoing progress in genomics, high-throughput screening, and directed evolution technologies. Advanced bioprocess engineering provide more opportunities for applying enzyme technologies to meet chiral challenges in synthetic chemistry.