文献类型：期刊文献

英文题名：Physically Cross-Linked Hydrogels Based on Poly(vinyl alcohol) and Gelatin for Independent Modulation of Mechanical Cues in Cell Adhesion Studies

作者：Feng, Chen[1];Chen, Meng[1];Qu, Da-Hui[1]

机构：[1]East China Univ Sci & Technol, Key Lab Adv Mat & Joint Int Res Lab Precis Chem &, Frontiers Sci Ctr Materiobiol & Dynam Chem, Sch Chem & Mol Engn,Feringa Nobel Prize Scientist, Shanghai 200237, Peoples R China

年份：2026

卷号：9

期号：6

起止页码：3029

外文期刊名：ACS APPLIED BIO MATERIALS

收录：EI(收录号：20261220290343);WOS:【ESCI(收录号:WOS:001694425100001)】；

基金：We thank the Research Center of Analysis and Test of East China University of Science and Technology for the help with the characterization.

语种：英文

外文关键词：hydrogels; mechanical property; cell adhesion; physical cross-linking; poly(vinyl alcohol); gelatin

摘要：Mechanical properties of biomaterials constitute a key parameter in regulating cellular responses. However, isolating the mechanical influence on cell behavior remains challenging due to the interdependent changes in chemical composition (e.g., polymer chain concentration or type, cross-linking agent, etc.) when modifying a single variable. Herein, noncovalently cross-linked hydrogels comprising poly(vinyl alcohol) (PVA) and gelatin (GEL) with gradient mechanical strength are fabricated by controlling the total freezing-thawing repetitions applied to the PVA-GEL mixture, in which the mechanical properties of hydrogels are successfully decoupled from the chemical composition for cell adhesion studies. The L929 cells prefer the stiff PVA-GEL hydrogel surfaces over the soft ones, uncovering significant variations in cellular attachment and spreading patterns. This strategy may provide a basis for systematically investigating the contribution of specific cues to cellular adhesion processes.