文献类型：期刊文献

英文题名：Kinetic study of the degradation of forskolin in aqueous systems by stability-indicating HPLC method and identification of its degradation products

作者：Wang, Yaqin[1];Yin, Jiajun[1];Weng, Weiyu[2];Liu, Zhenjun[2];Huang, Jianming[1]

机构：[1]Fudan Univ, Sch Pharm, Dept Pharmacognosy, Shanghai 201203, Peoples R China;[2]E China Univ Sci & Technol, Sch Pharm, Dept Pharmaceut, Shanghai 200237, Peoples R China

年份：2016

卷号：39

期号：1

起止页码：44

外文期刊名：JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES

收录：;EI(收录号：20160902042300);WOS:【SCI-EXPANDED(收录号:WOS:000373691600007)】；

基金：Financial support from the Science and Technology Commission of the Shanghai Municipality (13401900502) is gratefully acknowledged.

语种：英文

外文关键词：Degradation kinetics; forskolin; forskolin D; HPLC; isoforskolin; stability

摘要：The degradation kinetics of forskolin in aqueous solution was investigated qualitatively and quantitatively. Two degradation products were isolated and identified as isoforskolin and forskolin D by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and nuclear magnetic resonance (NMR) spectroscopy. A stability-indicating high-performance liquid chromatography (HPLC) method was developed and validated for the quantification of forskolin and its degradation products. Chromatographic separation was performed on a Luna C-18 column with acetonitrile-water (65: 35, v/v) as the mobile phase. The flow rate was kept at 1 mL/min, and the detection wavelength was 210 nm. The kinetic study of forskolin was carried out in aqueous solutions of pH 1.5-8.5 at 37, 50, 65, and 80 degrees C. The degradation rate of forskolin increases with increasing temperature. Forskolin is relatively stable in the pH range 3.5-6.5, but its stability decreases when the pH is outside this range. In the pH range 6.5-8.5, the forskolin degradation follows pseudo-first-order kinetics. Based on the structural identification and quantitative analysis of the degradation products, a possible pathway for forskolin degradation is proposed. Forskolin can be converted to isoforskolin rapidly, and both forskolin and isoforskolin can further decompose to forskolin D. [GRAPHICS] .