文献类型：期刊文献

英文题名：Heterologous production of α-farnesene in metabolically engineered strains of Yarrowia lipolytica

作者：Yang, Xia[1];Nambou, Komi[2];Wei, Liujing[1];Hua, Qiang[1,2]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Shanghai Collaborat Innovat Ctr Biomfg, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2016

卷号：216

起止页码：1040

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20162602541367);WOS:【SCI-EXPANDED(收录号:WOS:000379555900129)】；

基金：This work was funded by the National Basic Research Program of China (973 Program) (2012CB721101), National Natural Science Foundation of China (21576089).

语种：英文

外文关键词：Biofuels; Fed-batch; Metabolic engineering; Terpene synthases; Yarrowia lipolytica

摘要：Herein, we studied the heterologous production of alpha-farnesene, a valuable sesquiterpene with various biotechnological applications, by metabolic engineering of Yarrowia lipolytica. Different overexpression vectors harboring combinations of tHMG1, IDI, ERG20 and codon-optimized alpha-farnesene synthase (OptFS) genes were constructed and integrated into the genome of Y. lipolytica Po1h. The engineered strain produced 57.08 +/- 1.43 mg/L of alpha-farnesene corresponding to 20.8-fold increase over the initial production of 2.75 +/- 0.29 mg/L in the YPD medium in shake flasks. Bioreactor scale-up in PM medium led to alpha-farnesene concentration of 259.98 +/- 2.15 mg/L with alpha-farnesene to biomass ratio of 33.98 +/- 1.51 mg/g, which was a 94.5-fold increase over the initial production. This first report on alpha-farnesene synthesis in Y. lipolytica lays a foundation for future research on production of sesquitepenes in Y. lipolytica and other closest yeast species and will potentially contribute in its industrial production. (C) 2016 Elsevier Ltd. All rights reserved.