文献类型：期刊文献

英文题名：Highly Selective Fluorescent Probe for Vicinal-Dithiol-Containing Proteins and In Situ Imaging in Living Cells

作者：Huang, Chusen[1];Yin, Qin[1];Zhu, Weiping[1];Yang, Yi[1];Wang, Xin[1];Qian, Xuhong[1];Xu, Yufang[1]

机构：[1]E China Univ Sci & Technol, Sch Pharm, State Key Lab Bioreactor Engn, Shanghai Key Lab Chem Biol, Shanghai, Peoples R China

年份：2011

卷号：50

期号：33

起止页码：7551

外文期刊名：ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

收录：;EI(收录号：20113214227409);WOS:【SCI-EXPANDED(收录号:WOS:000294092100014)】；

基金：We are grateful for financial support from the National Natural Science Foundation of China (grants 90713026, 21076077), the National Basic Research Program of China (973 Program, 2010CB126100), the National High Technology Research and Development Program of China (863 Program, 2011AA10A207), the China 111 Project (grant B07023), the Fok Ying Tung Education Foundation (grant No. 111022), the Shanghai Leading Academic Discipline Project (B507), and the Fundamental Research Funds for the Central Universities. We also thank Prof. Dr. Charles Yang for the improvement of this article.

语种：英文

外文关键词：fluorescent probes; in situ imaging; protein labeling; redox proteomics; vicinal dithiols

摘要：It pays to be direct: In a rapid and specific approach to the detection of vicinal-dithiol-containing proteins (VDPs), the use of a fluorescent probe (see picture) enabled the direct readout of fluorescence. This approach based on fluorescence polarization, electrophoresis, and the direct imaging of VDPs permits the noninvasive study of VDPs both in vitro and in living cells and offers insight into their potential roles in cell function. Copyright ? 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.