文献类型：期刊文献

英文题名：Chaetoglobosins and azaphilones from Chaetomium globosum associated with Apostichopus japonicus

作者：Qi, Jun[1];Jiang, Lan[2];Zhao, Peipei[1];Chen, Haiyan[3];Jia, Xiaopeng[1];Zhao, Liya[1];Dai, Huanqin[6,7];Hu, Jiansen[6,7];Liu, Changheng[1];Shim, Sang Hee[4,5];Xia, Xuekui[1];Zhang, Lixin[1,2]

机构：[1]Qilu Univ Technol, Shandong Acad Sci, Inst Biol, Jinan 250103, Shandong, Peoples R China;[2]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[3]Guangxi Univ, Coll Med, Guangxi Coll & Univ Key Lab Appl Chem Technol & R, Nanning 530004, Peoples R China;[4]Duksung Womens Univ, Coll Pharm, Seoul 01369, South Korea;[5]Duksung Womens Univ, Innovat Drug Ctr, Seoul 01369, South Korea;[6]Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100101, Peoples R China;[7]Chinese Acad Sci, Inst Microbiol, CAS Key Lab Pathogen Microbiol & Immunol, Beijing 100101, Peoples R China

年份：2020

卷号：104

期号：4

起止页码：1545

外文期刊名：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

收录：;EI(收录号：20200207994200);WOS:【SCI-EXPANDED(收录号:WOS:000505363500002)】；

基金：Funding was provided by the National Natural Science Foundation of China (No. 31770024), the Natural Science Foundation from Shandong Province (No. ZR2017MH025, ZR2017ZB0206), National Research Foundation of Korea, (NRF-2019R1A4A1020626), the Taishan Scholar Project from Shandong Province to Lixin Zhang.

语种：英文

外文关键词：Apostichopus japonicus; Chaetomium globosum; Genome mining; Chaetoglobosins; Azaphilones; Antimicrobial activity

摘要：Increasing attention has recently been focused on complex symbiotic associations, for instance coral and its symbionts. Sea cucumber, harboring diverse fungi, has also attracted more and more attention for their functional diversity. Here, secondary metabolites produced by Chaetomium globosum associated with sea cucumber, Apostichopus japonicus, were investigated using gene mining with third-generation sequencing technology (PacBio SMRT). Nine compounds, including one new compound cytoglobosin X (1), were isolated from cultures of Chaetomium globosum. Compound 1 was identified based on NMR data, HRESIMS, and ECD, and the absolute configurations were identified as 3S, 4R, 7S, 8R, 9R, 16S, 19S, 20S, and 23S. In an antimicrobial assay, compound 4 showed moderate activity against Staphylococcus aureus and methicillin-resistant Staphylococcus aureus with MICs of 47.3 and 94.6 mu M, respectively. Our results suggest that the microbiomes associated with sea cucumber could be an important resource for biodiversity and structural novelty, and the bioactive compounds may protect the host from pathogen microbial.