详细信息
耐有机溶剂脂肪酶产生菌的筛选和鉴定
The Screening and Identification of a Novel Strain Producing an Organic Solvent-Tolerant Lipase
文献类型:期刊文献
中文题名:耐有机溶剂脂肪酶产生菌的筛选和鉴定
英文题名:The Screening and Identification of a Novel Strain Producing an Organic Solvent-Tolerant Lipase
作者:彭仁[1,2];林金萍[1];魏东芝[1]
机构:[1]华东理工大学反应器工程国家重点实验室,上海200237;[2]江西师范大学生命科学学院,江西南昌330022
年份:2011
卷号:35
期号:2
起止页码:197
中文期刊名:江西师范大学学报(自然科学版)
外文期刊名:Journal of Jiangxi Normal University(Natural Science Edition)
收录:CSTPCD;;北大核心:【北大核心2008】;
基金:国家重点基础研究发展计划(2009CB724703);江西省科技支撑计划(200800361)资助项目
语种:中文
中文关键词:铜绿假单胞菌;有机溶剂耐受性;脂肪酶
外文关键词:Pseudomonas aeruginosa; organic solvents tolerance; lipase
摘要:从国内各地采取土样分离筛选产耐有机溶剂脂肪酶的新菌株,通过形态和生理生化特征以及系统进化树分析,筛选到的新菌株命名为Pseudomonas aeruginosa CS-2.来自Pseudomonas aeruginosa CS-2的粗脂肪酶液在乙腈中酶活提高,在苯、氯仿、正己烷、石油醚和异辛烷中表现出较高的稳定性.
A novel strain which produced an organic solvent-tolerant lipase was isolated from soils in different areas of China.The strain was identified as Pseudomonas aeruginosa CS-2 by morphological,physiological and biochemical features and phylogenetic tree analysis.The crude lipase was activated in the presence of acetonitrile and it exhibited high stability in presence of benzene,chloroform,n-Hexane,petroleum ether and isooctane.
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