文献类型：期刊文献

英文题名：A highly integrated DNA nanomachine operating in living cells powered by an endogenous stimulus

作者：Ma, Pei-Qiang[1,2];Liang, Cheng-Pin[2];Zhang, He-Hua[2];Yin, Bin-Cheng[1,2];Ye, Bang-Ce[1,2]

机构：[1]Zhejiang Univ Technol, Coll Pharmaceut Sci, Collaborat Innovat Ctr Yangtze River Delta Reg Gr, Hangzhou 310014, Zhejiang, Peoples R China;[2]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Lab Biosyst & Microanal, Shanghai 200237, Peoples R China

年份：2018

卷号：9

期号：13

起止页码：3299

外文期刊名：CHEMICAL SCIENCE

收录：;EI(收录号：20181404979120);WOS:【SCI-EXPANDED(收录号:WOS:000431113300005)】；

基金：This work was jointly supported by the National Natural Science Foundation of China (Grants 21335003, 21675052, and 21575089), the Fundamental Research Funds for the Central Universities, and the Programme of Introducing Talents of Discipline to Universities (Grant B16017).

语种：英文

外文关键词：Additives - RNA - Cytology - Gold nanoparticles - Cells

摘要：Synthetic molecular machines have received increasing attention because of their great ability to mimic natural biological motors and create novel modes of motion. However, very few examples have been implemented with real autonomous movement inside living cells, due to the challenges of the driving force and highly integrated system design. In this work, we report an elegant, highly integrated DNA nanomachine that can be powered by endogenous ATP molecules and autonomously operated inside living cells without any auxiliary additives. It assembles all components on a single gold nanoparticle (AuNP) including a hairpin-locked swing arm encoding a start triggered by an intracellular target molecule and a two-stranded DNA track responding to the motion of the swing arm. When the intracellular target activates the nanomachine via the unlocking swing arm, the machine autonomously and progressively operates on the established DNA track via intramolecular toehold-mediated strand migration and internal ATP binding. This paper also demonstrates the machine's bioanalytical application for specific microRNA (miRNA) imaging in living cells.