文献类型：期刊文献

英文题名：Characterization of CRISPR Loci and Antimicrobial Resistance in Foodborne Listeria monocytogenes Isolates

作者：Wu, Yufan[1];Huang, Xiaoqiang[2];Gu, Qiang[3];Li, Yun[2];Zhou, Yuan[2];Sun, Lu[2];Wang, Xiang[2]

机构：[1]East China Univ Sci & Technol, Ctr Anal & Test, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[2]Univ Shanghai Sci & Technol, Sch Hlth Sci & Engn, Shanghai 200093, Peoples R China;[3]Technol Ctr Zhangjiagang Customs, Suzhou 215600, Peoples R China

年份：2026

卷号：14

期号：7

外文期刊名：MICROORGANISMS

收录：;Scopus(收录号：2-s2.0-105045884108);WOS:【SCI-EXPANDED(收录号:WOS:001832865000001)】；

基金：This research was funded by the Scientific Research Project of Nanjing Customs (2025KJ29).

语种：英文

外文关键词：CRISPR arrays; antimicrobial resistance; foodborne isolates; Listeria monocytogenes

摘要：Clustered regularly interspaced short palindromic repeats (CRISPR) are widespread in bacterial and archaeal genomes as an adaptive immune system against invading mobile genetic elements. This study investigated the distribution of CRISPR loci and their potential association with antimicrobial resistance (AMR) in 40 foodborne Listeria monocytogenes isolates. CRISPR analysis showed that 18 isolates harbored CRISPR Locus 1, five carried Locus 2, and five possessed both loci. Antimicrobial susceptibility testing against seven antimicrobial agents indicated that most isolates were highly susceptible to the tested agents. Specifically, all isolates were susceptible to gentamicin, ampicillin, penicillin, and tetracycline, whereas resistance was observed in a small subset of isolates: four were resistant to chloramphenicol, five to levofloxacin, and four to ciprofloxacin. Statistical analysis showed no statistically significant association between the presence of CRISPR loci and antimicrobial susceptibility phenotypes. These findings provide baseline information on CRISPR locus distribution and antimicrobial susceptibility profiles in foodborne L. monocytogenes isolates. Further studies based on larger isolate collections, whole-genome sequencing, and characterization of associated cas genes are needed to clarify the potential role of CRISPR-Cas systems in AMR evolution in this species.