文献类型：期刊文献

英文题名：Development of a continuous cell-recycle fermentation system for production of lactic acid by Lactobacillus paracasei

作者：Xu, Guo-qian; Chu, Ju; Wang, Yong-hong; Zhuang, Ying-ping; Zhang, Si-liang; Peng, Hua-qiong

机构：[1]E China Univ Sci & Technol, Natl Engn Ctr Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2006

卷号：41

期号：12

起止页码：2458

外文期刊名：PROCESS BIOCHEMISTRY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000242259100016)】；

语种：英文

外文关键词：electromagnetic flow-meter; Lactobacillus paracasei; lactic acid; tangential velocity; membrane bioreactor; cell-recycle

摘要：Experimental devices for stimulating productivity of lactic acid fermentation were installed and an electromagnetic flow-meter and a pneumatic diaphragm pump were employed to alleviate the fouling of the membrane and to improve the durability of membrane cell-recycle bioreactors (MCRBs). In this case, the continuous fermentation using a 5 L automatic fermentor lasted stably over 150 h, and could repeat periodically with simple intermittent on-line cleaning and sterilization of the membrane filtration system. Maximum value of OD620 of 98.7 was obtained, six times greater than that of the fed-batch fermentation. Meanwhile, maximum productivity of 31.5 g/(L h) was recorded, 10 times greater than that occurred in the fed-batch fermentation. Compared with conventional MCRBs, the MCRB system with a diaphragm pump and tangential flow-rate controlling was more stable and durable. The tangential velocity of membrane module could be monitored and controlled on-line, and the possibility of contamination due to hose rupture could be eliminated. (c) 2006 Elsevier Ltd. All rights reserved.