文献类型：期刊文献

英文题名：Driven Translocation of Polynucleotides Through an Aerolysin Nanopore

作者：Cao, Chan;Yu, Jie;Wang, Ya-Qan;Ying, Yi-Lun;Long, Yi-Tao[1]

机构：[1]E China Univ Sci & Technol, Key Lab Adv Mat, Shanghai 200237, Peoples R China; E China Univ Sci & Technol, Dept Chem, Shanghai 200237, Peoples R China

年份：2016

卷号：88

期号：10

起止页码：5046

外文期刊名：ANALYTICAL CHEMISTRY

收录：;EI(收录号：20162402479210);WOS:【SCI-EXPANDED(收录号:WOS:000376223500006)】；

基金：This work was supported by the National Basic Research Program of China (973 Program, Grant No. 2013CB733700) and the National Natural Science Foundation of China (Grant Nos. 21421004 and 21327807). Y.-T.L. is supported by the Chang Jiang Scholars Program.

语种：英文

外文关键词：Nucleotides - Proteins - Refractive index

摘要：Aerolysin has been used as a biological nanopore for studying peptides, proteins, and oligosaccharides in the past two decades. Here, we report that wild-type aerolysin could be utilized for polynucleotide analysis. Driven a short polynucleotide of four nucleotides length through aerolysin occludes nearly 50% amplitude of the open pore current. Furthermore, the result of total internal reflection fluorescence measurement provides direct evidence for the driven translocation of single polynucleotide through aerolysin.