文献类型：期刊文献

英文题名：Sequential Nucleophilic Substitutions Permit Orthogonal Click Functionalization of Multicomponent PEG Brushes

作者：Sha, Jin[1,4];Lippmann, Ethan S.[1,2];McNulty, Jason[1,3];Ma, Yulu[4];Ashton, Randolph S.[1,2]

机构：[1]Univ Wisconsin, Wisconsin Inst Discovery, Madison, WI USA;[2]Univ Wisconsin, Dept Biomed Engn, Madison, WI USA;[3]Univ Wisconsin, Dept Mech Engn, Madison, WI 53706 USA;[4]E China Univ Sci & Technol, Sch Mech & Power Engn, Shanghai 200237, Peoples R China

年份：2013

卷号：14

期号：9

起止页码：3294

外文期刊名：BIOMACROMOLECULES

收录：;EI(收录号：20133816754945);WOS:【SCI-EXPANDED(收录号:WOS:000330095500038)】；

基金：The authors acknowledge Prof. Nicholas Abbott for the generous use of his AFM and PM-IRRAS. This work was supported by funds from the University of Wisconsin-Madison and the Wisconsin Institute for Discovery.

语种：英文

外文关键词：Triethanolamine - Cell culture - Synthesis (chemical) - Lighting - Sodium Azide - Ethanolamines - Ligands - Polyethylene glycols - Surface treatment

摘要：Multicomponent poly(ethylene glycol) (PEG) brushes (i.e., >= 2 adjacent PEG brushes) can be used to engineer culture substrates with microscale, nonfouling regions decorated with covalently immobilized ligands that mediate biospecific interactions. However, synthesizing such brushes with orthogonal immobilization chemistries to permit differential biofunctionalization is nontrivial and often requires synthesis of PEG-co-polymers. To simplify synthesis and enhance the versatility of such substrates, we developed a protocol for generating orthogonal click-functionalized multicomponent PEG brushes using sequential nucleophilic substitutions by sodium azide, ethanolamine, and propargylamine. The novel application of propargylamine-mediated substitution functionalizes PEG brushes with acetylene groups, and for the first time, ethanolamine-mediated substitution is shown to be sufficient for passivating the "living" polymer chain ends between brush synthesis steps. Thus, our multicomponent PEG brushes present dual orthogonal chemistries (i.e., azido and acetylene groups) for ligand immobilization via versatile copper-free click reactions, which are useful for in situ surface modifications during cell culture.