文献类型：期刊文献

中文题名：高速逆流色谱法分离制备沉香中的沉香四醇和4′-甲氧基沉香四醇

英文题名：Preparative isolation and purification of agarotetrol and 4′-methoxyagarotetrol from Aquilaria sinensis (Lour.) Gilg by high-speed counter-current chromatography

作者：郑克敏[1];张继全[2];沈平孃[2];卓超[1]

机构：[1]华东理工大学药学院,上海200237;[2]国家中药制药工程技术研究中心,上海201203

年份：2011

卷号：33

期号：1

起止页码：96

中文期刊名：中成药

外文期刊名：Chinese Traditional Patent Medicine

收录：CSTPCD;;北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

基金：国家科技部国际科技合作项目--戒毒中药"济泰片"方剂的配伍规律及防复吸的机理研究(2006DFA32720)

语种：中文

中文关键词：高速逆流色谱;沉香;沉香四醇;4′-甲氧基沉香四醇;2-(2-苯乙基)色酮

外文关键词：high-speed counter-current chromatography； Aquilaria sinensis（Lour.） Gilg； agarotetrol； 4′-me-thoxyagarotetrol； 2-（2-Phenylethyl）chromone；

摘要：目的:研究高速逆流色谱分离制备沉香中2-(2-苯乙基)色酮类活性成分的方法。方法:采用氯仿-甲醇-水(4∶2.6∶2.4)为两相溶剂体系,上相为固定相,流速1.2 mL/min,正向转速900 rpm。结果:利用高速逆流色谱法从沉香95%乙醇粗提物中一次性分离得到两个2-(2-苯乙基)色酮衍生物——沉香四醇(agarotetrol,AH1)和4′-甲氧基沉香四醇。所得两分离峰经EI-MS、1H-NMR和13C-NMR鉴定确认。HPLC检测纯度可达99.0%以上。结论:高速逆流色谱法对于沉香中结构相近的2-(2-苯乙基)色酮衍生物具有理想的分离效果,产物纯度高。
AIM：To establish the method of separating 2-（2-Phenylethyl）chromone derivatives from agalwood by high-speed counter-current chromatography（HSCCC）.METHODS：Chloroform-methanol-water（4：2.6：2.4,v/v/v） was used as the two-phase solvent system.The upper phase was used as the stationary phase,the revolution speed was 900 rpm and the flow rate was 1.2 mL/min.RESULTS：A preparative HSCCC method was successfully established for separating agarotetrol and 4′-methoxyagarotetrol from 95% ethanol extract of Aquilaria sinensis（Lour.）Gilg.Identification of two constituents was carried out according to its EI-MS、1H-NMR and 13C-NMR da-ta.The purity of agarotetrol and 4′-methoxyagarotetrol was 99.0%,as determined by HPLC.CONCLUSION：HSCCC is successfully used for the separation and purification of two main 2-（2-Phenylethyl）chromone derivatives which has similar structure from agalwood with ideal purity.