文献类型：期刊文献

英文题名：A graphene aptasensor for biomarker detection in human serum

作者：Wang, Xuejun[1,2];Zhu, Yibo[2];Olsen, Timothy R.[2];Sun, Na[3];Zhang, Wenjun[1,4];Pei, Renjun[3];Lin, Qiao[2]

机构：[1]East China Univ Sci & Technol, Dept Mech Engn, Shanghai 200237, Peoples R China;[2]Columbia Univ, Dept Mech Engn, New York, NY 10027 USA;[3]Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou 215123, Jiangsu, Peoples R China;[4]Univ Saskatchewan, Dept Mech Engn, Saskatoon, SK S7N 5A9, Canada

年份：2018

卷号：290

起止页码：356

外文期刊名：ELECTROCHIMICA ACTA

收录：;EI(收录号：20184105938274);WOS:【SCI-EXPANDED(收录号:WOS:000447008700039)】；

基金：This work was supported by the National Science Foundation, United States (Grant No. ECCS-1509760) and National Institutes of Health (Grant No. 1R33CA196470-01A1). X. Wang gratefully acknowledges a National Scholarship (Award No. 201406740003) from the China Scholarship Council. The authors would also like to thank Dr. Tilla S. Worgall at the Department of Pathology and Cell Biology, Columbia University, for providing human serum used in the experiments.

语种：英文

外文关键词：Affinity sensing; Biomarker detection; Graphene; Nonspecific binding; Human serum

摘要：This paper presents an affinity graphene nanosensor for detection of biomarkers in undiluted and non-desalted human serum. The affinity nanosensor is a field-effect transistor in which graphene serves as the conducting channel. The graphene surface is sequentially functionalized with a nanolayer of the polymer polyethylene glycol (PEG) and a biomarker-specific aptamer. The aptamer is able to specifically bind with and capture unlabeled biomarkers in serum. A captured biomarker induces a change in the electric conductivity of the graphene, which is measured in a buffer of optimally chosen ionic strength to determine the biomarker concentration. The PEG layer effectively rejects nonspecific adsorption of background molecules in serum while still allowing the aptamer to be readily accessible to serum-borne biomarkers and increases the effective Debye screening length on the graphene surface. Thus, the aptamer-biomarker binding sensitively changes the graphene conductivity, thereby achieving specific and label-free detection of biomarkers with high sensitivity and without the need to dilute or desalt the serum. Experimental results demonstrate that the graphene nanosensor is capable of specifically capturing human immunoglobulin E (IgE), used as a representative biomarker, in human serum in the concentration range of 50 pM-250 nM, with a resolution of 14.5 pM and a limit of detection of 47 pM. (C) 2018 Elsevier Ltd. All rights reserved.