文献类型：期刊文献

英文题名：Methanogenic biodegradation of C9 to C12n-alkanes initiated by Smithella via fumarate addition mechanism

作者：Ji, Jia-Heng[1,2];Zhou, Lei[1,2];Mbadinga, Serge Maurice[1,2];Irfan, Muhammad[1,2,3];Liu, Yi-Fan[1,2];Pan, Pan[1,2];Qi, Zhen-Zhen[1,2];Chen, Jing[1,2];Liu, Jin-Feng[1,2];Yang, Shi-Zhong[1,2];Gu, Ji-Dong[4];Mu, Bo-Zhong[1,2,5]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Chem & Mol Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[3]Univ Engn & Technol, Dept Chem Polymer & Composite Mat Engn, KSK Campus, Lahore 54890, Pakistan;[4]Univ Hong Kong, Sch Biol Sci, Pokfulam Rd, Hong Kong, Peoples R China;[5]East China Univ Sci & Technol, Engn Res Ctr Microbial Enhanced Oil Recovery, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2020

卷号：10

期号：1

外文期刊名：AMB EXPRESS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000511569800001)】；

基金：This work was supported by the National Natural Science Foundation of China (Grants Nos. 41530318, 41403066), NSFC/RGC Joint Research Fund (No. 41161160560), the Fundamental Research Funds for the Central Universities (Nos. 222201817017, 50321101917017, 22221818014) and the Research Program of State Key Laboratory of Bioreactor Engineering.

语种：英文

外文关键词：Alkanes; Alkylsuccinates; Fumarate addition; Methanogenesis; Oil reservoirs

摘要：In the present study, a methanogenic alkane-degrading (a mixture of C-9 to C(12)n-alkanes) culture enriched from production water of a low-temperature oil reservoir was established and assessed. Significant methane production was detected in the alkane-amended enrichment cultures compared with alkane-free controls over an incubation period of 1 year. At the end of the incubation, fumarate addition metabolites (C-9 to C-12 alkylsuccinates) and assA genes (encoding the alpha subunit of alkylsuccinate synthase) were detected only in the alkane-amended enrichment cultures. Microbial community analysis showed that putative syntrophic n-alkane degraders (Smithella) capable of initiating n-alkanes by fumarate addition mechanism were enriched in the alkane-amended enrichment cultures. In addition, both hydrogenotrophic (Methanocalculus) and acetoclastic (Methanothrix) methanogens were also observed. Our results provide further evidence that alkanes can be activated by addition to fumarate under methanogenic conditions.