文献类型：期刊文献

英文题名：Oxidation of organic electron donor by denitratation: Performance, pathway and key microorganism

作者：Li, Wei[1,2,6];Liu, Shuai[3];Zhang, Meng[1];Zhao, He-Ping[1,4];Zheng, Ping[1,4,5]

机构：[1]Zhejiang Univ, Coll Environm & Resource Sci, Dept Environm Engn, Hangzhou, Zhejiang, Peoples R China;[2]East China Univ Sci & Technol, Sch Resources & Environm Engn, State Environm Protect Key Lab Environm Risk Asse, Shanghai, Peoples R China;[3]Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Beijing, Peoples R China;[4]Zhejiang Univ, Zhejiang Prov Key Lab Water Pollut Control & Envi, Hangzhou, Zhejiang, Peoples R China;[5]Zhejiang Univ, Coll Environm & Resource Sci, MOE Key Lab Environm Remediat & Ecosyst Hlth, Hangzhou, Zhejiang, Peoples R China;[6]Sun Yat Sen Univ, Guangdong Prov Key Lab Environm Pollut Control &, Guangzhou, Guangdong, Peoples R China

年份：2018

卷号：343

起止页码：554

外文期刊名：CHEMICAL ENGINEERING JOURNAL

收录：;EI(收录号：20181104908462);WOS:【SCI-EXPANDED(收录号:WOS:000430269200058)】；

基金：This work was financially supported by Natural Science Foundation of China (Nos. 51578484, 51608474, 41601243), China Postdoctoral Science Foundation China (No. 2016M590540), Fundamental Research Funds for the Central Universities (222201814053) and Research Fund Program of Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology (2018K16). Thanks to Prof. James Shapleigh from Cornell for helping improved the English through whole manuscript.

语种：英文

外文关键词：Nitrogen bioremoval; Organic electron donor; Denitratation; Enzymatic assay; Metagenomics

摘要：In this paper, two classical organic electron donors, i.e. acetate and glucose, were chosen to study the oxidation of organics coupling with denitratation enrichment culture via batch tests and to predicate its key functional bacteria via genomes-based metagenomics. The results showed that acetate-denitratation was observed to have better denitratation performances (nitrate reduction rate of 13.5-18.5 mg.g(-1)VSS.h(-1) and nitrite yield of 96.2%) than glucose-denitratation (nitrate reduction rate of 4.6-6.5 mg.g(-1)VSS.h(-1) and nitrite yield of 71.5%). Distinctive but preferable acetogenesis (acetate production) step was observed in glucose-fed test, which resulted in the decline of denitratation performance by lower intracellular NADH/NAD(+) ratio. The enzymatic assay of key carbon metabolism indicated that the low NADH/NAD(+) ratio was likely impacted by up-regulating the acetate generation from glucose and down-regulating the acetate utilization. Metagenomic binning and phylogenetic analysis revealed that a new Halomonas genome (only shared an average nucleotide identify of 83% to the most related species) dominated in the system, and was most likely responsible for this kind of acetogenesis in denitratation. The findings of this work are important for both the development of denitratation-based nitrogen removal technology and the insight into fundamental microbial metabolism of nitrogen coupling carbon oxidation.