文献类型：期刊文献

英文题名：A single-component light sensor system allows highly tunable and direct activation of gene expression in bacterial cells

作者：Li, Xie[1,2];Zhang, Changcheng[1,2];Xu, Xiaopei[1,2];Miao, Jun[1,2];Yao, Jing[1,2];Liu, Renmei[1,2];Zhao, Yuzheng[1,2];Chen, Xianjun[1,2,3,4];Yang, Yi[1,2,3]

机构：[1]East China Univ Sci & Technol, Optogenet & Synthet Biol Interdisciplinary Res Ct, Shanghai Collaborat Innovat Ctr Biomfg Technol, State Key Lab Bioreactor Engn, 130 Mei Long Rd, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, 130 Mei Long Rd, Shanghai 200237, Peoples R China;[3]Chinese Acad Sci, CAS Ctr Excellence Brain Sci, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China;[4]East China Univ Sci & Technol, 130 Mei Long Rd, Shanghai 200237, Peoples R China

年份：2020

卷号：48

期号：6

外文期刊名：NUCLEIC ACIDS RESEARCH

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000525957500003)】；

基金：National Key Research and Development Program of China [2017YFA050400 to Y.Y. and 2019YFA0904800 to Y.Z.]; NSFC [21937004, 91857202 to Y.Y., 31600688 and 31971349 to X.C., 31900833 to X.L.]; Shanghai Science and Technology Commission [18JC1411900 to Y.Y., 19ZR1472800 to X.C.]; China Postdoctoral Science Foundation [2016M59027 and 2017T00277 to X.C., 2019M651414 to X.L.]; State Key Laboratory of Bioreactor Engineering (to Y.Y.); Fundamental Research Funds for the Central Universities (to Y.Y. and X.C.); 111 Project [B07023]. Funding for open access charge: National Key Research and Development Program of China [2017YFA050400].

语种：英文

摘要：Light-regulated modules offer unprecedented new ways to control cellular behaviour with precise spatial and temporal resolution. Among a variety of bacterial light-switchable gene expression systems, single-component systems consisting of single transcription factors would be more useful due to the advantages of speed, simplicity, and versatility. In the present study, we developed a single-component light-activated bacterial gene expression system (eLightOn) based on a novel LOV domain from Rhodobacter sphaeroides (RsLOV). The eLightOn system showed significant improvements over the existing single-component bacterial light-activated expression systems, with benefits including a high ON/OFF ratio of >500-fold, a high activation level, fast activation kinetics, and/or good adaptability. Additionally, the induction characteristics, including regulatory windows, activation kinetics and light sensitivities, were highly tunable by altering the expression level of LexRO. We demonstrated the usefulness of the eLightOn system in regulating cell division and swimming by controlling the expression of the FtsZ and CheZ genes, respectively, as well as constructing synthetic Boolean logic gates using light and arabinose as the two inputs. Taken together, our data indicate that the eLightOn system is a robust and highly tunable tool for quantitative and spatiotemporal control of bacterial gene expression.