文献类型：期刊文献

英文题名：Evidence of single-nanoparticle translocation through a solid-state nanopore by plasmon resonance energy transfer

作者：Cao, Yue[1,2];Lin, Yao[1,2];Qian, Ruo-Can[1,2];Ying, Yi-Lun[1,2];Si, Wei[3];Sha, Jingjie[3];Chen, Yunfei[3];Long, Yi-Tao[1,2]

机构：[1]E China Univ Sci & Technol, Key Lab Adv Mat, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Dept Chem, Shanghai 200237, Peoples R China;[3]Southeast Univ, Sch Mech Engn, Jiangsu Key Lab Design & Fabricat Micronano Biome, Nanjing 211189, Jiangsu, Peoples R China

年份：2016

卷号：52

期号：30

起止页码：5230

外文期刊名：CHEMICAL COMMUNICATIONS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000373687400002)】；

基金：This research was supported by the National Natural Science Foundation of China (21421004, 21327807, 51375092, 21505043), the 57th Chinese Post-doctor Fund (2015M570335, 2015M570336) and the Program of Introducing Talents of Discipline to Universities (B16017).

语种：英文

摘要：This work proposes a gold nanoparticle (AuNP) based probe to study the single-nanoparticle translocation behavior through a silicon nitride (SiNx) solid-state nanopore. The AuNP probe is functionalized with a rhodamine derivative molecule, termed Rhod-DPA, whose fluorescence can be activated in the presence of Cu2+ due to the binding between Rhod-DPA and Cu2+. The Cu2+ triggered configuration change of Rhod-DPA on the probe surface can induce the plasmon resonance energy transfer (PRET) from single AuNPs to the transformed fluorescent molecules, which can be detected by the color change of AuNP probes under dark-field microscopy (DFM) and their scattering spectra recorded on a spectrometer. By analyzing the peak shifts before and after the addition of Cu2+, evidence of single nanoparticle translocation through the nanopore has been obtained, proving the successful establishment of the tracking strategy.