文献类型：期刊文献

中文题名：双龙方有效成分诱导干细胞分化过程中差异表达基因的筛选和聚类分析

英文题名：Screening and Cluster of Differentially Expressed MSCs Genes Induced by Shuanglong Prescription

作者：王金津[1];钱夕元[1];李雪[2];杨辉华[2];梁琼麟[2];王义明[2];罗国安[2]

机构：[1]华东理工大学理学院,上海200237;[2]清华大学中药现代化研究中心,北京100084

年份：2007

卷号：9

期号：3

起止页码：39

中文期刊名：世界科学技术-中医药现代化

外文期刊名：Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

收录：CSTPCD

基金：科学技术部"十一五"科技支撑计划项目(2006BAI08B04-01):中药复方作用和配伍评价研究;科学技术部973计划专项课题(2005CB523503):中医临床防治慢性肾功能衰竭的系统生物学研究

语种：中文

中文关键词：双龙方;基因芯片;差异基因表达;hierarchical聚类分析

外文关键词：Shuanglong Prescription; Gene chip; differentially expressed gene; hierarchical cluster method

摘要：目的:利用基因芯片筛选双龙方主要成分(人参皂苷Rb1,丹参酚酸B)诱导大鼠骨髓间充质干细胞(MSCs)分化过程中的差异表达基因,并对差异表达基因进行聚类分析,研究双龙方主要有效成分对大鼠MSCs增殖及分化的影响。方法:用含5705条大鼠基因的博奥大鼠全基因组Oligo芯片,以不同给药情况、不同时间点的细胞总RNA制备的探针杂交芯片,用LuxScan10KA双通道激光扫描仪(CapitalBio公司)进行扫描,筛选MSCs变化过程中的差异表达基因并进行生物信息分析,hierarchical聚类提取差异表达的特征基因。结果:在MSCs的分化过程中,筛选出128条(2.24%)差异表达基因,经分析发现它们与能量代谢,信号传导等多类基因密切相关,对样本进行聚类发现MSCs在20-30天之间发生了显著的改变。结论:基因芯片是研究中药作用机理的有效平台,双龙方主要成分可诱导MSCs向心肌样细胞分化。
To investigate the effect of Shuanglong prescription components on MSCs by using gene chips to screening and cluster the differentially expressed MSCs genes induced by Shuanglong Prescription. METHODS： BOAO rat microarrays （include 5705 rat genes） were used to prepare gene chips hybrided by cell total RNA of different conditions. The chips were scanned with LuxScan 10KA dual channels laser scanner of CapitalBio Company to look for the information of differently expressed genes. RESULTS： 128 differently expressed genes（2.24% ）were screened out from the 5705 target genes, which were found to be correlated with energy metabolism and signal transduction. Bioinformatics analysis suggests that MSCs changed remarkably in the period of 20 to 30 days. CONCLUSIONS： DNA microarray is an effective platform for the study and analysis of mechanisms of TCM. It can be concluded that Shuanglong Prescription components can induce MSCs to differentiate into cardiomyocytes.