文献类型：期刊文献

中文题名：杂交瘤细胞培养的优化

英文题名：Optimization in Hybridoma Cell Culture

作者：张元兴[1,2];方宏勋[1,2];容秉培[1,2]

机构：[1]华东理工大学生物反应器工程国家重点实验室;[2]香港城市大学生物及化学系

年份：1998

卷号：14

期号：3

起止页码：326

中文期刊名：生物工程学报

外文期刊名：Chinese Journal of Biotechnology

收录：CSTPCD;;Scopus;北大核心:【北大核心1996】；CSCD:【CSCD2011_2012】；PubMed;

语种：中文

中文关键词：杂交瘤;细胞培养;醋酸钾;灌注培养

外文关键词：Cell culture,hybridoma,perfusion culture,potassium acetate,optimization

摘要：根据杂交瘤细胞培养中单克隆抗体生产的动力学原理，采用灌注培养方式、添加醋酸钾和丰富营养物培养的途径，对反应器放大培养过程进行了优化。每天灌注１／２反应器工作体积，与分批培养相比，细胞密度由４５×１０５／ｍｌ提高到１１×１０５／ｍｌ，单抗浓度由１９ｍｇ／Ｌ提高到２８ｍｇ／Ｌ；添加１ｇ／Ｌ醋酸钾，细胞密度基本保持不变，但单抗浓度增加到３８μｇ／ｍｌ；用丰富营养物培养后，细胞密度和单抗浓度分别进一步提高到４２×１０５／ｍｌ和９４ｍｇ／Ｌ。抗Ｂ型红细胞单抗的血凝滴度，由分批培养的１：３２，最终提高到１：２５６。
Based on the formation kinetics of monoclonal antibody,the hybridoma cell culture in bioreactor was optimized by the application of perfusion culture mode,the supplementation of potassium acetate,and the fortification of nutrients.When the bioreactor was operated with the daily perfusion of medium of 1/2 work volume,viable cell density in bioreactor was ,in comparison with batch culture,increased from 4.5×10 5cells/ml to 11×10 5cells/ml and antibody concentration in supernatant from 19mg/L to 28mg/L.After supplemented with 1g/L potassium acetate,the cell density kept constant and antibody production was improved to 38mg/L.The fortification of nutrients such as amino acids and vitamins raised the cell density to 42×10 5cells/ml and ,responsively,antibody concentration to 94mg/L.