文献类型：期刊文献

英文题名：Exploring Verrucosidin Derivatives with Glucose-Uptake-Stimulatory Activity from Penicillium cellarum Using MS/MS-Based Molecular Networking

作者：Han, Junjie[1];Chen, Baosong[1];Zhang, Rui[2];Zhang, Jinjin[1];Dai, Huanqin[1];Wang, Tao[1];Sun, Jingzu[1];Zhu, Guoliang[3];Li, Wei[1];Li, Erwei[4];Liu, Xueting[3];Yin, Wenbing[1];Liu, Hongwei[1,2]

机构：[1]Chinese Acad Sci, Inst Microbiol, State Key Lab Mycol, Beijing 100101, Peoples R China;[2]Shenyang Pharmaceut Univ, Sch Med Devices, Shenyang 110016, Peoples R China;[3]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[4]Chinese Acad Sci, Inst Ctr Shared Technol & Facil, Inst Microbiol, Beijing 100101, Peoples R China

年份：2022

卷号：8

期号：2

外文期刊名：JOURNAL OF FUNGI

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000773925700001)】；

基金：This project was supported by the National Special Project for Key Science and Technology of Food Safety (grant No. 2017YFC1601302), the National Key R & D Program of China (grant No. 2017YFE0108200), and the National Natural Science Foundation (Grant Nos. 22177131, and 82073723).

语种：英文

外文关键词：verrucosidins; Penicillium cellarum; glucose uptake-stimulating activity; molecular networking

摘要：Under the guidance of LC-MS/MS-based molecular networking, seven new verrucosidin derivatives, penicicellarusins A-G (3-9), were isolated together with three known analogues from the fungus Penicillium cellarum. The structures of the new compounds were determined by a combination of NMR, mass and electronic circular dichroism spectral data analysis. The absolute configuration of penicyrone A (10) was corrected based on X-ray diffraction analyses. Bioactivity screening indicated that compounds 1, 2, and 4 showed much stronger promising hypoglycemic activity than the positive drug (rosiglitazone) in the range of 25-100 mu M, which represents a potential new class of hypoglycemic agents. Preliminary structure-activity relationship analysis indicates that the formation of epoxy ring on C-6-C-7 in the structures is important for the glucose uptake-stimulating activity. The gene cluster for the biosynthesis of 1-12 is identified by sequencing the genome of P. cellarum and similarity analysis with the gene cluster of verrucosidins in P. polonicum.