文献类型：期刊文献

英文题名：Characterization of Streptomyces sp. LS462 with high productivity of echinomycin, a potent antituberculosis and synergistic antifungal antibiotic

作者：Chen, Caixia[1,7];Chen, Xiangyin[2];Ren, Biao[1,3];Guo, Hui[1,4];Abdel-Mageed, Wael M.[1,5];Liu, Xueting[1,2];Song, Fuhang[1,6];Zhang, Lixin[1,2]

机构：[1]Chinese Acad Sci, Inst Microbiol, Beijing 100101, Peoples R China;[2]East China Univ Sci & Technol, Sch Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[3]Sichuan Univ, West China Hosp Stomatol, Chengdu 610041, Sichuan, Peoples R China;[4]Chinese Acad Sci, Inst Biophys, Beijing 100190, Peoples R China;[5]King Saud Univ, Coll Pharm, Dept Pharmacognosy, POB 2457, Riyadh 11451, Saudi Arabia;[6]Beijing Technol & Business Univ, Sch Light Ind, Beijing 100048, Peoples R China;[7]Univ Pittsburgh, Sch Med, Pittsburgh, PA 15213 USA

年份：2021

卷号：48

期号：9-10

外文期刊名：JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

收录：;EI(收录号：20242616415295);WOS:【SCI-EXPANDED(收录号:WOS:000893028400002)】；

语种：英文

外文关键词：Streptomyces fuscichromogenes; Echinomycin; Antituberculosis; Synergistic antifungal activity; Posaconazole

摘要：A biologically active microbial strain, designated as "LS462," was isolated from a soil sample collected from Yaoli Virgin Forest of Jiangxi Province, China. The strain was able to produce a high yield of echinomycin (172 mg/l) even under nonoptimized culture conditions and is proposed to serve as a promising source of echinomycin. In this study, echinomycin exhibited strong anti-Mycobacterium tuberculosis H37Rv activity and synergistic antifungal effect with a greatly reduced dosage of posaconazole on Candida albicans SC5314. The strain belongs to the genus Streptomyces according to its morphological and 16S rDNA phylogenetic analysis. The 16S rDNA was found to have the highest sequence identity with Streptomyces fuscichromogenes (99.37% similarity). Extensive nuclear magnetic resonance and mass spectroscopic data were used to determine the structure of echinomycin. The strain S. fuscichromogenes has not been previously reported to produce echinomycin. Strain LS462 may be exploited as a new potential source for the commercial production of echinomycin. Also, this work is the first to report the new synergistic antifungal activity of echinomycin and further study of the synergistic mechanism will be helpful to guide the development of antifungal agents.