文献类型：期刊文献

英文题名：Induced effect of Ca2+ on dalesconols A and B biosynthesis in the culture of Daldinia eschscholzii via calcium/calmodulin signaling

作者：Lu, Yanhua[1]; Pan, Zhenghua[1]; Tao, Jun[1]; An, Faliang[1]

机构：[1] State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China

年份：2018

卷号：125

期号：2

起止页码：205

外文期刊名：Journal of Bioscience and Bioengineering

收录：EI(收录号：20174104269983)

语种：英文

外文关键词：Signaling - Biosynthesis - Biochemistry - Chlorine compounds - Calmodulin - Genes

摘要：Dalesconols (dalesconols A and B) were isolated from Daldinia eschscholzii and have remarkable immunosuppressive activity. In this study, the response of fungal growth, intra- and extracellular Ca2+, and dalesconols production after CaCl2 addition were reported for the first time. After supplementation with 5 mM Ca2+ at 24 h, dalesconols production reached 84.33 mg/L, which resulted in a 1.57-fold enhancement compared to the control. The key role of calcium/calmodulin signaling in dalesconols biosynthesis was confirmed by treatment with Ca2+ channel and calmodulin inhibitors. The transcriptional levels of dalesconols biosynthetic genes were up-regulated after CaCl2 addition and down-regulated after inhibitors were added. The results demonstrated that Ca2+ addition induces dalesconols biosynthesis through up-regulation of dalesconols biosynthesis genes via regulation of calcium/calmodulin signaling. This study provided an efficient strategy for improving dalesconols production and would facilitate further research on the biosynthesis and regulation of dalesconols. ? 2017 The Society for Biotechnology, Japan