文献类型：期刊文献

中文题名：Fabrication and evaluation of a BMP-2/dexamethasone co-loaded gelatin sponge scaffold for rapid bone regeneration

作者：Qi Gan[1,2];Hao Pan[3];Wenjing Zhang[3];Yuan Yuan[1];Jiangchao Qian[2];Changsheng Liu[1,3,4]

机构：[1]Key Laboratory for Ultrafine Materials of Ministry of Education,East China University of Science and Technology,Shanghai 200237,PR China;[2]The State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai 200237,PR China;[3]Engineering Research Center for Biomedical Materials of the Ministry of Education,East China University of Science and Technology,Shanghai 200237,PR China;[4]Frontiers Science Center for Materiobiology and Dynamic Chemistry,East China University of Science and Technology,Shanghai 200237,PR China

年份：2022

卷号：9

期号：1

起止页码：303

中文期刊名：Regenerative Biomaterials

外文期刊名：再生生物材料（英文版）

收录：Scopus;PubMed

基金：supported by the Natural Science Foundation of China for Innovative Research Groups(No.51621002);the National Natural Science Foundation of China(No.32071337);Shanghai Pujiang Program(20PJ1402600);the 111 Project(B14018);supported by‘the Fundamental Research Funds for the Central Universities’.

语种：英文

中文关键词：BMP-2;dexamethasone;Runx2;pre-clinical;bone regeneration

摘要：Improving the osteogenic activity of BMP-2 in vivo has significant clinical application value.In this research,we use a clinical gelatin sponge scaffold loaded with BMP-2 and dexamethasone(Dex)to evaluate the osteogenic activity of dual drugs via ectopic osteogenesis in vivo.We also investigate the mechanism of osteogenesis induced by BMP-2 and Dex with C2C12,a multipotent muscle-derived progenitor cell.The results show that the gelatin scaffold with Dex and BMP-2 can significantly accelerate osteogenesis in vivo.It is indicated that compared with the BMP-2 or Dex alone,100nM of Dex can dramatically enhance the BMP-2-induced alkaline phosphatase activity(ALP),ALP mRNA expression and mineralization.Further studies show that 100nM of Dex can maintain the secondary structure of BMP-2 and facilitate recognition of BMP-2 with its receptors on the surface of C2C12 cells.We also find that in C2C12,Dex has no obvious effect on the BMP-2-induced Smad1/5/8 protein expression and the STAT3-dependent pathway,but Runx2-dependent pathway is involved in the Dex-stimulated osteoblast differentiation of BMP-2 both in vitro and in vivo.Based on these results,a potential mechanism model about the synergistic osteoinductive effect of Dex and BMP-2 in C2C12 cells via Runx2 activation is proposed.This may provide a theoretical basis for the pre-clinical application of Dex and BMP-2 for bone regeneration.