文献类型：期刊文献

英文题名：Analysis of alkane-dependent methanogenic community derived from production water of a high-temperature petroleum reservoir

作者：Mbadinga, Serge Maurice[1,2];Li, Kai-Ping[1,2];Zhou, Lei[1,2];Wang, Li-Ying[1,2];Yang, Shi-Zhong[1,2];Liu, Jin-Feng[1,2];Gu, Ji-Dong[3];Mu, Bo-Zhong[1,2]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Inst Appl Chem, Shanghai 200237, Peoples R China;[3]Univ Hong Kong, Sch Biol Sci, Hong Kong, Hong Kong, Peoples R China

年份：2012

卷号：96

期号：2

起止页码：531

外文期刊名：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

收录：;EI(收录号：20123915468709);WOS:【SCI-EXPANDED(收录号:WOS:000308953100023)】；

基金：This work was supported by the National Natural Science Foundation of China (grant no. 51174092) and the 863 Program (grant no. 2009AA063503).

语种：英文

外文关键词：Alkylsuccinate synthase; High-temperature petroleum reservoir; Hydrogenotrophic methanogenesis; Methanogenic degradation of alkanes; Syntrophic acetate oxidation; Microbial community

摘要：Microbial assemblage in an n-alkanes-dependent thermophilic methanogenic enrichment cultures derived from production waters of a high-temperature petroleum reservoir was investigated in this study. Substantially higher amounts of methane were generated from the enrichment cultures incubated at 55 A degrees C for 528 days with a mixture of long-chain n-alkanes (C-15-C-20). Stoichiometric estimation showed that alkanes-dependent methanogenesis accounted for about 19.8% of the total amount of methane expected. Hydrogen was occasionally detected together with methane in the gas phase of the cultures. Chemical analysis of the liquid cultures resulted only in low concentrations of acetate and formate. Phylogenetic analysis of the enrichment revealed the presence of several bacterial taxa related to Firmicutes, Thermodesulfobiaceae, Thermotogaceae, Nitrospiraceae, Dictyoglomaceae, Candidate division OP8 and others without close cultured representatives, and Archaea predominantly related to uncultured members in the order Archaeoglobales and CO2-reducing methanogens. Screening of genomic DNA retrieved from the alkanes-amended enrichment cultures also suggested the presence of new alkylsuccinate synthase alpha-subunit (assA) homologues. These findings suggest the presence of poorly characterized (putative) anaerobic n-alkanes degraders in the thermophilic methanogenic enrichment cultures. Our results indicate that methanogenesis of alkanes under thermophilic condition is likely to proceed via syntrophic acetate and/or formate oxidation linked with hydrogenotrophic methanogenesis.