文献类型：期刊文献

英文题名：Unlocking monosaccharide composition of capsular polysaccharide and lipopolysaccharide to combat Pseudomonas aeruginosa

作者：Huang, Qingchun[1];Wang, Hongye[1];Zhao, Yanjun[1];Cai, Danni[2];Luan, Shaorong[2];Xiao, Ciying[3]

机构：[1]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Sch Biotechnol, Shanghai 200237, Peoples R China

年份：2025

卷号：334

外文期刊名：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

收录：;EI(收录号：20254719549934);WOS:【SCI-EXPANDED(收录号:WOS:001625449000005)】；

基金：This work was in part supported by the National Natural Science Foundation of China (32372588) and the project of Science and Technology Plan of Shanghai Municipal in China (21S11901400).

语种：英文

外文关键词：Monosaccharide; Capsular polysaccharide; Lipopolysaccharide; Ion chromatography; Pseudomonas aeruginosa

摘要：The pathogen Pseudomonas aeruginosa produces capsular polysaccharide (CPS) and lipopolysaccharide (LPS) as key pathogenic factors. However, the responses of their monosaccharide compositions to antibacterial agents remain poorly understood. This study characterized and compared the monosaccharide composition and proportion in CPS and LPS of P. aeruginosa strains ATCC 9027 and ATCC 15442 after treatment with a polysaccharide modulator, quinazolin-6-yl isoindolinone (IQE-X1). Monosaccharide levels were analyzed using optimized ion-exchange chromatography. The antibacterial activity, biofilm suppression, and cellulase inhibition of IQE-X1 were also assessed. Results showed that CPS and LPS from both strains contained eight monosaccharides: rhamnose, arabinose, glucosamine, galactose, glucose, xylose, mannose, and ribose, with strain-specific abundance profiles. ATCC 9027 was rich in ribose, while ATCC 15442 had high glucose and ribose in CPS, and high ribose and glucosamine in LPS. Neither strain contained trehalose, galacturonic acid, or glucuronic acid. IQE-X1 exhibited antibacterial activity, with IC50 values of 0.39-4.12 mu g/mL for ATCC 9027 and 0.36-7.97 mu g/mL for ATCC 15442 after 16 h of treatment. It also inhibited 99.5 % of biofilm formation and 82.4 % of cellulase activity in ATCC 9027. IQE-X1 reduced all monosaccharide levels in the CPS of both strains, with distinct reduction patterns for each strain. Furthermore, it altered LPS composition: ATCC 9027 showed increased glucosamine and mannose levels, while ATCC 15442 exhibited a significant decrease in arabinose, galactose, and glucose. These findings reveal strain-specific monosaccharide profiles in CPS and LPS and highlight a potential strategy to combat P. aeruginosa by modulating monosaccharide composition and proportion.